resumen
Abstract
Leptospirosis is a zoonotic disease with significant public health and economic impact worldwide. Rapid and accurate diagnosis is essential for effective prevention and treatment. This study optimized a loop-mediated isothermal amplification (LAMP) assay using BFo isothermal DNA polymerase with different colorimetric indicators. LAMP was able to detect DNA from pathogenic and intermediate leptospires, while non-pathogenic leptospires and other
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dc.contributor.author | Hamer, Micaela | |
dc.contributor.author | Watanabe, Olivia | |
dc.contributor.author | Saraullo, Vanina Rosa | |
dc.contributor.author | Ortega, Facundo Miguel | |
dc.contributor.author | Sanchez, Maria Cristina | |
dc.contributor.author | Martinez, Mara Leila | |
dc.contributor.author | Brihuega, Bibiana Felicitas | |
dc.contributor.author | Grune Loffler, Sylvia | |
dc.date.accessioned | 2023-09-15T12:38:41Z | |
dc.date.available | 2023-09-15T12:38:41Z | |
dc.date.issued | 2024-03 | |
dc.identifier.issn | 1573-7446 | |
dc.identifier.other | https://doi.org/10.1007/s11259-023-10183-1 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/15227 | |
dc.identifier.uri | https://link.springer.com/article/10.1007/s11259-023-10183-1 | |
dc.description.abstract | Leptospirosis is a zoonotic disease with significant public health and economic impact worldwide. Rapid and accurate diagnosis is essential for effective prevention and treatment. This study optimized a loop-mediated isothermal amplification (LAMP) assay using BFo isothermal DNA polymerase with different colorimetric indicators. LAMP was able to detect DNA from pathogenic and intermediate leptospires, while non-pathogenic leptospires and other non-leptospiral microorganisms were negative. LAMP assay combined with calcein showed a tenfold higher limit of detection (1 ng of leptospiral DNA per reaction) than LAMP combined with hydroxynaphthol blue or end-point PCR lipL32 (10 ng of DNA per reaction). Animal samples were collected from infected and non-infected Golden Syrian hamsters (Mesocricetus auratus) to evaluate and compare the performance of LAMP and PCR. These techniques showed a substantial agreement according to Cohen’s kappa statistic, being both useful techniques for detecting leptospiral DNA in clinical samples. Overall, this study demonstrates that the LAMP assay is a sensitive, specific, rapid, and simple tool for the detection of leptospiral DNA. It has the potential to facilitate the diagnosis of leptospirosis, particularly in low-income regions with limited diagnosis resources. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Springer | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | Veterinary Research Communications : 103-111 (2024) | es_AR |
dc.subject | Animal Models | eng |
dc.subject | Modelos Animales | es_AR |
dc.subject | Infectious Diseases | eng |
dc.subject | Enfermedades Infecciosas | es_AR |
dc.subject | Leptospira | eng |
dc.subject | PCR | eng |
dc.subject | Reacción en Cadena de la Polimerasa | es_AR |
dc.subject | Hamsters | eng |
dc.subject | Hámster | es_AR |
dc.subject.other | Loop-mediated Isothermal Amplification | eng |
dc.subject.other | Amplificación Isotérmica Mediada por Bucle | es_AR |
dc.subject.other | Sensitivity | eng |
dc.subject.other | Sensibilidad | es_AR |
dc.subject.other | Specificity | eng |
dc.subject.other | Especificidad | es_AR |
dc.title | Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | Instituto de Patobiología | es_AR |
dc.description.fil | Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina | es_AR |
dc.description.fil | Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina | es_AR |
dc.description.fil | Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina | es_AR |
dc.description.fil | Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Ortega, Facundo Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina | es_AR |
dc.description.fil | Fil: Ortega, Facundo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina | es_AR |
dc.description.fil | Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina | es_AR |
dc.description.fil | Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina | es_AR |
dc.description.fil | Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Escuela de Veterinaria; Argentina | es_AR |
dc.description.fil | Fil: Grune Loffler, Sylvia. Universidad Abierta Interamericana (UAI); Argentina | es_AR |
dc.description.fil | Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Virología Humana y Animal (CEVHAN); Argentina | es_AR |
dc.subtype | cientifico |
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