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Mostrando ítems 1-16 de 16
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Aplicación de la técnica PMA-qPCR para detectar viabilidad de Escherichia coli productor de toxina Shiga (STEC) en hamburguesas de carne vacuna.
(2019-11)El PMA (propidium monoazide) es un colorante fotoreactivo que se intercala en el ADN bacteriano de las células muertas e inhibe su amplificación. Su selectividad se fundamenta en que la membrana bacteriana es impermeable ... -
Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have Hypervirulent-Like phenotypes
(PLOS, 2015-06-01)The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a disease that mainly affects children under 5 years of age. Argentina is the country with the highest ... -
Evaluación de un ensayo de amplificación isotérmica mediada por bucles (LAMP) para detectar el gen stx2 en aislamientos clínicos de Escherichia coli en Argentina
(2015-09)Escherichia coli productor de toxina Shiga (STEC del inglés Shiga toxin-producing Escherichia coli) es un patógeno de transmisión alimentaria que puede producir enfermedades en humanos tales como diarreas hemorrágicas así ... -
Evaluación del efecto combinado de ácidos orgánicos y ultrasonido sobre la inactivación de Escherichia coli inoculado en hamburguesas de carne vacuna
(2019-11)Escherichia coli productor de toxina Shiga (STEC) es el agente causal de diarreas con o sin sangre y, en los casos más severos, Síndrome Urémico Hemolítico. La hamburguesa de carne vacuna ha sido descripta como uno de los ... -
Evaluación del efecto del tratamiento con alta presión hidrostática y de la formulación sobre la inactivación de cepas nativas de STEC O157 en hamburguesas de carne bovina
(INTA, 2018)El mercado actual ofrece al consumidor distintas formulaciones de hamburguesas reflejadas en productos tradicionales o más saludables (reducidos en contenidos de grasa y cloruro de sodio). En este contexto, el objetivo ... -
Frequency, characterization and genotypic analysis of Shiga toxin-producing Escherichia coli in beef slaughterhouses of Argentina = Frecuencia, caracterización y análisis genotípico de Escherichia coli productor de toxina Shiga en frigoríficos de carne bovina de Argentina
(2018-06-22)The objectives of this study were: (1) to estimate STEC frequency in hide and carcass samples taken from beef slaughterhouses supplying the domestic market in Argentina, (2) to establish the pheno-genotypic characteristics ... -
High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio
(Elsevier, 2014-10-22)High-pressure treatments (400 and 600 MPa) combined with the addition of sodium lactate (1 and 3%) were tested to reduce Escherichia coli O157:H7 (STEC O157) and spoilage microbiota contamination in a manufactured cured ... -
Immune response in calves vaccinated with type three secretion system antigens and shiga toxin 2B subunit of Escherichia coli O157:H7
(2017-01-03)Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine ... -
Immunization of pregnant cows with Shiga toxin-2 induces high levels of specific colostral antibodies and lactoferrin able to neutralize E. coli O157:H7 pathogenicity
(2018-03)E. coli O157:H7 is a foodborne pathogen responsible for bloody diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). The objective of the present work was to evaluate the ability of colostral IgG obtained from ... -
Novel Effector Protein EspY3 of Type III Secretion System from Enterohemorrhagic Escherichia coli Is Localized in Actin Pedestals
(MDPI AG, 2018)Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic Escherichia coli (EPEC) are attaching and effacing (A/E) pathogens, which translocate effector proteins to intestinal enterocytes through a type III secretion ... -
Overexpressed proteins in hypervirulent clade 8 and clade 6 strains of Escherichia coli O157:H7 compared to E. coli O157:H7 EDL933 clade 3 strain
(2016)Escherichia coli O157:H7 is responsible for severe diarrhea and hemolytic uremic syndrome (HUS), and predominantly affects children under 5 years. The major virulence traits are Shiga toxins, necessary to develop HUS and ... -
Risk factors for shiga toxin-producing Escherichia coli-associated human diseases
(American Society for Microbiology, 2014)We have reviewed the risk factors for the occurrence of Shiga toxin-producing Escherichia coli (STEC)-associated human diseases. The analysis of STEC surveillance data and trends shows differences in frequency and severity ... -
Secretion systems of pathogenic escherichia coli
(Springer International Publishing, 2016)Protein secretion plays a central role in modulating the interactions of bacteria with their environments. Bacterial ribosomes synthesize up to 8000 different proteins. Almost half of these become integrated in membranes ... -
Shiga toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces E. coli O157:H7 adherence in vitro and in vivo
(2017-09)Problem addressed: Shiga toxin-producing Escherichia coli (STEC) are a group of bacteria responsible for foodassociated diseases. Clinical features include a wide range of symptoms such as diarrhea, hemorrhagic colitis and ... -
Type VI secretion system in pathogenic escherichia coli: structure, role in virulence, and acquisition
(Frontiers Media, 2019-08)Bacterial pathogens utilize a myriad of mechanisms to invade mammalian hosts, damage tissue sites, and evade the immune system. One essential strategy of Gram-negative bacteria is the secretion of virulence factors through ... -
Validación de irradiación para reducir la presencia de Escherichia coli productor de toxina Shiga en carne picada fresca.
(2019-09)Escherichia coli productor de toxina Shiga (STEC) es el agente causal de diarreas con o sin sangre y, en los casos más severos, del Síndrome Urémico Hemolítico. La carne picada ha sido descripta como uno de los principales ...