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Abstract
This study aimed to evaluate the sequence variations of the GABA-Cl gene of Rhipicephalus (Boophilus) microplus ticks from Argentina with different resistance levels to fipronil. Genomic DNA was obtained from engorged females (n = 50) of fipronil-susceptible and resistant tick field populations from five localities in northern Argentina. Tick populations were tested for fipronil resistance by in vitro larval packet test. DNA encoding amino acid residues [ver mas...]
dc.contributor.authorSarli, Macarena
dc.contributor.authorTorrents, Jorgelina
dc.contributor.authorMorel, Nicolas
dc.contributor.authorNava, Santiago
dc.date.accessioned2022-09-15T10:29:43Z
dc.date.available2022-09-15T10:29:43Z
dc.date.issued2022-11
dc.identifier.citationMacarena Sarli, Jorgelina Torrents, Nicolás Morel, Santiago Nava, Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina, Ticks and Tick-borne Diseases, Volume 13, Issue 6, 2022, 102030.es_AR
dc.identifier.issn1877-959X
dc.identifier.otherhttps://doi.org/10.1016/j.ttbdis.2022.102030
dc.identifier.urihttp://hdl.handle.net/20.500.12123/12884
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1877959X22001327
dc.description.abstractThis study aimed to evaluate the sequence variations of the GABA-Cl gene of Rhipicephalus (Boophilus) microplus ticks from Argentina with different resistance levels to fipronil. Genomic DNA was obtained from engorged females (n = 50) of fipronil-susceptible and resistant tick field populations from five localities in northern Argentina. Tick populations were tested for fipronil resistance by in vitro larval packet test. DNA encoding amino acid residues 264–360 of the GABA-Cl was amplified by PCR. PCR products were sequenced and the translated amino acid sequences were aligned and compared among each other and with sequences of the GABA-Cl belonging to the Brazilian and Uruguayan reference strains fipronil-susceptible (Mozo) and resistant (RFSan and Juarez). All the GABA-Cl amino acid sequences obtained from the R. microplus specimens from Argentina were identical, regardless of the fipronil resistance-susceptibility status of each population. Mutations present in GABA-Cl sequence of RFSan and Juarez strains were not present in any of the populations from Argentina analyzed. These results indicate the failure of this gene as a molecular marker of resistance to fipronil, at least in the R. microplus populations from northern Argentina.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceTicks and Tick-borne Diseases 13 (6) : 102030 (November 2022)es_AR
dc.subjectRhipicephaluseng
dc.subjectBoophilus micropluseng
dc.subjectGamma-aminobutyric Acideng
dc.subjectÁcido Gamma-aminobutíricoes_AR
dc.subjectFipronileng
dc.subjectInsecticide Resistanceeng
dc.subjectResistencia a los Insecticidases_AR
dc.subjectArgentinaes_AR
dc.subject.otherGABA
dc.titleAnalysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentinaes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentinaes_AR
dc.description.filFil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentinaes_AR
dc.description.filFil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentinaes_AR
dc.description.filFil: Morel, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentinaes_AR
dc.description.filFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentinaes_AR
dc.description.filFil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentinaes_AR
dc.subtypecientifico


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