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Abstract
The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of [ver mas...]
dc.contributor.authorCamussone, Cecilia
dc.contributor.authorReidel, Ivana Gabriela
dc.contributor.authorMolineri, Ana Ines
dc.contributor.authorCicotello, Joaquin
dc.contributor.authorMiotti, Camila
dc.contributor.authorSuarez Archilla, Guillermo
dc.contributor.authorCurti, C.C.
dc.contributor.authorVeaute, Carolina Melania Isabel
dc.contributor.authorCalvinho, Luis Fernando
dc.dateinfo:eu-repo/date/embargoEnd/2023-03-07
dc.date.accessioned2022-03-07T14:02:23Z
dc.date.available2022-03-07T14:02:23Z
dc.date.issued2022-07
dc.identifier.issn0034-5288
dc.identifier.otherhttps://doi.org/10.1016/j.rvsc.2022.02.014
dc.identifier.urihttp://hdl.handle.net/20.500.12123/11331
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0034528822000510
dc.description.abstractThe aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherElsevieres_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública
dc.rightsinfo:eu-repo/semantics/embargoedAccesses_AR
dc.sourceResearch in Veterinary Science 145 : 177-187 (July 2022)es_AR
dc.subjectNovillaes_AR
dc.subjectHeiferseng
dc.subjectVacaes_AR
dc.subjectCowseng
dc.subjectStaphylococcus aureuses_AR
dc.subjectInmunizaciónes_AR
dc.subjectImmunizationeng
dc.subjectVacunaes_AR
dc.subjectVaccineseng
dc.subjectProteínas Recombinanteses_AR
dc.subjectRecombinant Proteinsemg
dc.subjectMastítis Bovina
dc.subjectBovine Mastitiseng
dc.titleEfficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cowses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/acceptedVersiones_AR
dc.description.origenEEA Rafaelaes_AR
dc.description.filFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentinaes_AR
dc.description.filFil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.es_AR
dc.description.filFil: Molineri, Ana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Cicotello, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Cicotello, Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Miotti, Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Miotti, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.description.filFil: Curti, C.C. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentinaes_AR
dc.description.filFil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.es_AR
dc.description.filFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentinaes_AR
dc.description.filFil: Calvinho, Luis Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes_AR
dc.subtypecientifico


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