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Resumen
In-vitro fertilization is a routine livestock-breeding technique widely used around the world. Several studies have reported the interaction of bovine viral-diarrhea virus (BVDV) with gametes and in-vitro-produced (IVP) bovine embryos. Since, gene expression in BVDV-infected IVP bovine embryos is scarcely addressed. The aim of this work was to evaluate the differential expression of genes involved in immune and inflammatory response. Groups of 20–25
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dc.contributor.author | González Altamiranda, Erika | |
dc.contributor.author | Arias, María E. | |
dc.contributor.author | Kaiser, German Gustavo | |
dc.contributor.author | Mucci, Nicolas Crescencio | |
dc.contributor.author | Odeon, Anselmo Carlos | |
dc.contributor.author | Felmer, Ricardo N. | |
dc.date.accessioned | 2022-01-13T12:51:15Z | |
dc.date.available | 2022-01-13T12:51:15Z | |
dc.date.issued | 2020-11 | |
dc.identifier.issn | 0301-4851 | |
dc.identifier.issn | 1573-4978 | |
dc.identifier.other | https://doi.org/10.1007/s11033-020-05958-7 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/11115 | |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs11033-020-05958-7 | |
dc.description.abstract | In-vitro fertilization is a routine livestock-breeding technique widely used around the world. Several studies have reported the interaction of bovine viral-diarrhea virus (BVDV) with gametes and in-vitro-produced (IVP) bovine embryos. Since, gene expression in BVDV-infected IVP bovine embryos is scarcely addressed. The aim of this work was to evaluate the differential expression of genes involved in immune and inflammatory response. Groups of 20–25 embryos on Day 6 (morula stage) were exposed (infected) or not (control) to an NCP-BVDV strain in SOF medium. After 24 h, embryos that reached expanded blastocyst stage were washed. Total RNA of each embryo group was extracted to determine the transcription levels of 9 specific transcripts related with antiviral and inflammatory response by SYBR Green real time quantitative (RT-qPCR). Culture media and an aliquot of the last embryos wash on Day 7 were analyzed by titration and virus isolation, respectively. A conventional PCR confirmed BVDV presence in IVP embryos. A significantly higher expression of interferon-α was observed in blastocysts exposed to NCP-BVDV compared to the controls (p < 0.05). In this study, the upregulation of INFα and TLR7 genes involved in inflammatory and immune response in BVDV-infected IVP bovine embryos is a new finding in this field. This differential expression suggest that embryonic cells could function in a manner like immune cells by recognizing and responding early to interaction with viral pathogens. These results provide new insights into the action of BVDV on the complex molecular pathways controlling bovine early embryonic development. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Springer | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Molecular Biology Reports 47 : 9959–9965 (2020) | es_AR |
dc.subject | Ganado Bovino | es_AR |
dc.subject | Cattle | eng |
dc.subject | Embriones Animales | es_AR |
dc.subject | Animal Embryos | eng |
dc.subject | Infección Experimental | es_AR |
dc.subject | Experimental Infection | eng |
dc.subject | Pestivirus de la Diarrea Bovina | es_AR |
dc.subject | Bovine Diarrhoa Pestivirus | eng |
dc.subject | Experimentación in Vitro | es_AR |
dc.subject | In Vitro Experimentation | eng |
dc.title | Upregulation of interferon-alpha gene in bovine embryos produced in vitro in response to experimental infection with noncytophatic bovine-viral-diarrhea virus | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Instituto de Virología | es_AR |
dc.description.fil | Fil: Gonzalez Altamiranda, Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. | es_AR |
dc.description.fil | Fil: González Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. | es_AR |
dc.description.fil | Fil: Arias, María E. Universidad de La Frontera. Facultad de Ciencias Agrícolas y Forestales. Laboratorio de Reproducción Centro de Biotecnologia Reproductiva CEBIOR-BIOREN; Chile | es_AR |
dc.description.fil | Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. | es_AR |
dc.description.fil | Fil: Kaiser, German Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. | es_AR |
dc.description.fil | Fil: Mucci, Nicolas Crescencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. | es_AR |
dc.description.fil | Fil: Mucci, Nicolas Crescencio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. | es_AR |
dc.description.fil | Fil: Odeón, Anselmo Carlos. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. | es_AR |
dc.description.fil | Fil: Felmer, Ricardo N. Universidad de La Frontera. Facultad de Ciencias Agrícolas y Forestales. Laboratorio de Reproducción Centro de Biotecnologia Reproductiva CEBIOR-BIOREN; Chile | es_AR |
dc.subtype | cientifico |
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