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Mostrando ítems 1-20 de 20
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Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato
(Nature Publishing Group, 2021-02-22)DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein ... -
Análisis del microbioma de insectos: identificación y caracterización de glicosil hidrolasas
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2018)Algunos insectos han desarrollado estrategias muy eficaces para utilizar sustratos lignocelulósicos como fuentes de energía. En termitas y algunos coleópteros, la digestión de la lignocelulosa es el resultado de la acción ... -
Aumento de actividad antioxidante a bebidas comerciales por el agregado de extractos de especies silvestres obtenidas por asistencia enzimática
(Universidad Nacional de Santiago del Estero, Facultad de Agronomía y Agroindustrias, 2022-04)Solidago chilensis (SC) y Dipsacus fullonum (DF) son especies vegetales que crecen en la región noroeste de la provincia de Buenos Aires y han sido utilizadas como medicinas por culturas ancestrales. La extracción asistida ... -
Chapter 5 - Strategies to improve enzyme performance: Effect of high pressure on the substrate and pressure-assisted reaction
(Elservier, 2023)The chapter presents different practical applications that can be classified under the general framework of the management and optimization of enzymatic reactions influenced by high-pressure processing (HPP). These enzymes ... -
Characterization of a Novel Thermostable Enzyme from Thermus sp. 2.9 with Phospholipase and Acyltransferase Activities
(Karger, 2018)Phospholipases are classified in different enzyme families according to the ester bond they cleave within phospholipids. The use of phospholipases in industrial processes has prompted the search for new enzymes with ... -
Efecto de enzimas exógenas sobre la fermentación y calidad nutricional de ensilaje de sorgo
(Escuela para Graduados Ing. Agr. Alberto Soriano, Facultad de Agronomía, Universidad de Buenos Aires, 2018)La ganadería del norte argentino se realiza dispersa en una gran diversidad de ecosistemas compuestos mayoritariamente por pastizales naturales, con una gran producción de biomasa primavero-estival de calidad nutricional ... -
Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)
(Wiley, 2020-08)The cotton boll weevil, Anthonomus grandis, is a major pest of cotton crops in South America. In this work, partial biochemical characterizations of (hemi) cellulases and pectinases activities in the digestive system (head‐ ... -
Evaluation of hydrothermal and alkaline pretreatment routes for xylooligosaccharides production from sugar cane bagasse using different combinations of recombinant enzymes
(Springer, 2023-10)Xylan is the most abundant constituent of hemicellulose fraction of lignocellulosic biomass. Short xylooligosaccharides (XOS), obtained via xylan hydrolysis, have well-known prebiotic and antioxidant properties that are ... -
Identificación y caracterización de Aspartil proteasas en Solanum tuberosum relacionadas a diferentes tipos de estrés
(Secretaria de Ciencia y Tecnología, Universidad Nacional de Mar del Plata, 2020-10)En este trabajo, realizamos un análisis del genoma de S. tuberosum para explorar las funciones potenciales de las Aspartil proteasas (AP, EC 3.4.23). Las APs son enzimas proteolíticas con funciones muy diversas que se ... -
Induction of stress defense response and quality retention in minimally processed peaches through the application of gamma irradiation treatments
(Elsevier, 2022-08-21)Consumers demand fruit and vegetable products "on the go" that maintain color, flavor, nutritional and bioactive compounds almost equal to fresh products. Irradiation represents an effective technology traditionally used ... -
Influence of a tropical grass (Brachiaria brizantha cv. Mulato) as cover crop on soil biochemical properties in a degraded agricultural soil
(Elsevier, 2017-11)The inclusion of tropical grass forage as a cover crop (CC) could be a useful tool to improve microbiological activity and, consequently, soil quality. The aim of this study was to evaluate the effect of Brachiaria brizantha ... -
New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
(Elsevier, 2021-04)Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, ... -
Obtención de extractos vegetales a partir de especies silvestres asistida por enzimas carbohidrolasas
(Asociación Argentina de Tecnólogos Alimentarios - AATA, 2019-11)La utilización de enzimas carbohidrolasas es una metodología innovadora para la obtención de compuestos activos en extractos vegetales. Estas enzimas tienen un rango óptimo de temperatura entre 25-55 °C y actúan disgregando ... -
Obtención de plantas de papa sobrexpresantes de la aspartil proteasa StAP3 para incrementar la tolerancia a sequía
(Secretaria de Ciencia y Tecnología, Universidad Nacional de Mar del Plata, 2020-10)Una de las consecuencias del cambio climático es el estrés generado por la disminución de disponibilidad de agua (Leakey et al., 2009). La planta de papa, que representa una de las fuentes alimenticias más importantes para ... -
Optimization of high hydrostatic pressure processing for the preservation of minimally processed peach pieces
(2016)Consumers demand fresh-cut fruits, free from additives and with fresh appearance. However, the alteration caused by the tissue processing limits their shelf life. The aim of this work was to optimize the pressure level ... -
Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes
(Oxford Academic Press, 2011-02)The use of modified atmospheres has been successfully applied in different fruits to delay the ripening process and to prevent physiological disorders. In addition, during normal ripening, hypoxic areas are generated inside ... -
Relationship between microbial functions and community structure following agricultural intensification in South American Chaco
(Czech Academy of Agricultural Sciences, 2016-07)Intensification of agricultural systems through the use of intensive agriculture and the advance of deforestation have led to a decrease of soil biological quality. Soil functional and structural microbiota are sensitive ... -
ROS-Scavenging Enzymes as an Antioxidant Response to High Concentration of Anthracene in the Liverwort Marchantia polymorpha L.
(MDPI, 2021-07)Marchantia polymorpha L. responds to environmental changes using a myriad set of physiological responses, some unique to the lineage related to the lack of a vascular- and root-system. This study investigates the physiological ... -
Structural and molecular dynamics investigations of ligand stabilization via secondary binding site interactions in Paenibacillus xylanivorans GH11 xylanase
(Elsevier, 2021-03)Glycoside hydrolases (GHs) are essential for plant biomass deconstruction. GH11 family consist of endo-β-1,4-xylanases which hydrolyze xylan, the second most abundant cell wall biopolymer after cellulose, into small ... -
Structure of the novel monomeric glyoxalase I from Zea mays
(International Union of Crystallography, 2015-10)The glyoxalase system is ubiquitous among all forms of life owing to its central role in relieving the cell from the accumulation of methylglyoxal, a toxic metabolic byproduct. In higher plants, this system is upregulated ...