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An indirect ELISA validated as diagnostic and vaccines quality control tool for bovine parainfluenza virus TYPE 3 (BPIV3)
(CNRS (Francia), 2021-07)Serological assays, including enzyme-linked immunosorbent assays (ELISA), provide a useful tool to screen animals for the presence of antibodies (Abs) against a wide range of infectious agents (including viruses that cause ... -
Antibody detection against Kunitz-type protein in Fasciola hepatica experimentally infected sheep using enzyme-linked immunosorbent assay (ELISA)
(Taylor & Francis, 2023-11-14)Fasciolosis is a parasitic disease considered as emerging and neglected by the WHO. Sheep are highly susceptible to this disease, and affected flocks experience decreased productivity due to increased mortality, and the ... -
Asociación entre la densidad óptica y hallazgos post mortem compatibles con tuberculosis en bovinos positivos al test de ELISA
(Asosiación Argentina de Veterinarios de Laboratorios de Díagnóstico, 2021-11)Introducción. La tuberculosis bovina (TB) es una enfermedad infecto-contagiosa causada principalmente por Mycobacterium bovis. Genera un impacto económico negativo en la producción pecuaria y también en la salud pública, ... -
Assessment of the Efficiency of Technological Processes to Modify Whey Protein Antigenicity
(Multidisciplinary Digital Publishing Institute (MDPI), 2023-09-07)Whey is a by-product that represents a cheap source of protein with a high nutritional value, often used to improve food quality. When used as a raw material to produce hypoallergenic infant formulas (HIF), a processing ... -
Comparación de ELISAs comerciales para la detección de anticuerpos en la investigación diagnóstica del aborto asociado a Neospora caninum en rodeos lecheros de Uruguay
(Elsevier, 2020-06)A pesar de su importancia, el diagnóstico etiológico del aborto en bovinos representa un desafío técnico para los veterinarios clínicos y laboratoristas, en parte debido a la dificultad de recuperar los fetos abortados en ... -
Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis
(Elsevier, 2022-01)Leptospirosis is one of the most widely distributed zoonosis in the world. Bovine leptospirosis is a serious problem in bovine production, causing reproductive losses. The aim of this work was to compare recombinant LipL32 ... -
Descripción de la evolución de tuberculosis en rodeos lecheros combinando el uso de IDR y ELISA
(Asosiación Argentina de Veterinarios de Laboratorios de Díagnóstico, 2021-11)La tuberculosis bovina (TB) produce un impacto negativo en la producción pecuaria y la salud pública. La respuesta inmune a la infección es predominantemente mediada por células. En ello se fundamenta el diagnóstico a ... -
Detección y monitoreo de virus en colecciones de frutales de carozo en la provincia de Buenos Aires = Detection and virus monitoring in stone fruit pomological collections in Buenos Aires province
(2018)Para mejorar la calidad del material de propagación de frutales de carozo, la EEA San Pedro inició un programa de diagnóstico y monitoreo de sus colecciones, con el objeto de conocer su condición sanitaria con respecto ... -
Detection of recombinant human lactoferrin and lysozyme produced in a bitransgenic cow
(2017-03)Lactoferrin and lysozyme are 2 glycoproteins with great antimicrobial activity, being part of the nonspecific defensive system of human milk, though their use in commercial products is difficult because human milk is ... -
Determinación de la concentración de glifosato en agua mediante la técnica de inmunoabsorción ligada a enzimas (ELISA)
(2016)Los herbicidas constituyen fuentes potenciales de contaminación, siendo el agua uno de los componentes del ambiente más vulnerables. El glifosato (N-fosfonometil glicina) es el herbicida más utilizado a escala mundial. ... -
Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
(Elsevier, 2020-02)Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving ... -
Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia
(Springer, 2023-04)Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of ... -
Development and evaluation of a double-antigen sandwich ELISA to identify Anaplasma marginale–infected and A. centrale–vaccinated cattle
(American Association of Veterinary Laboratory Diagnosticians, 2019-11)Bovine anaplasmosis is a worldwide infectious disease caused by the intraerythrocytic bacterium Anaplasma marginale, which is transmitted by ticks and fomites. A. centrale is a less virulent subspecies used as a live ... -
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-19
(Elsevier, 2022-01)Serology tests for SARS-CoV-2 have proven to be important tools to fight against the COVID-19 pandemic. These serological tests can be used in low-income and remote areas for patient contact tracing, epidemiologic studies ... -
Development and field evaluation of an ELISA to differentiate Anaplasma marginale–infected from A. centrale–vaccinated cattle
(American Association of Veterinary Laboratory Diagnosticians, 2023-03)Immunization of calves with Anaplasma centrale is used to prevent acute anaplasmosis caused by A. marginale. Natural and vaccine-acquired immunity is detected through serologic tests based primarily on A. marginale recombinant ... -
Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
(Annex Publishers, 2019-08-26)Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several ... -
Development of a novel fusion protein with Anaplasma marginale and A. centrale MSP5 improved performance of Anaplasma antibody detection by cELISA in infected and vaccinated cattle
(Plos One, 2019-01-23)Detection of antibodies to Anaplasma spp. using commercial competitive enzyme-linked immunosorbent assay (ccELISA) is based on the recombinant major surface protein 5 fused to maltose binding protein (MBP-MSP5) or glutathione ... -
Development of an IgY-Based treatment to control bovine Coronavirus diarrhea in dairy calves
(MDPI, 2023-03)Bovine Coronavirus (BCoV) is a major pathogen associated with neonatal calf diarrhea. Standard practice dictates that to prevent BCoV diarrhea, dams should be immunized in the last stage of pregnancy to increase BCoV-specific ... -
Development of an Indirect ELISA Based on a Recombinant Chimeric Protein for the Detection of Antibodies against Bovine Babesiosis
(2018)The current method for Babesia spp. serodiagnosis based on a crude merozoite antigen is a complex and time-consuming procedure. An indirect enzyme-linked immunosorbent assay (iELISA) based on a recombinant multi-antigen ... -
Development of nanobodies against Mal de Río Cuarto virus major viroplasm protein P9‑1 for diagnostic sandwich ELISA and immunodetection
(Springer Nature, 2021-10)Mal de Río Cuarto virus (MRCV) is a member of the genus Fijivirus of the family Reoviridae that causes a devastating disease in maize and is persistently and propagatively transmitted by planthopper vectors. Virus replication ...