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Assessing Tn5 and sleeping beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes
Bevacqua, Romina Jimena; Fernández y Martin, Rafael; Canel, Natalia Gabriela; Gibbons, Alejandro Eduardo; Texeira, D.I.A.; Lange, F.; Vans Landschoot, Geraldina; Savy, Virginia; Briski, Olinda; Hiriart, María Inés; Grueso, Esther; Ivics, Zoltán; Taboga, Oscar Alberto; Kues, Wilfried A.; Ferraris, S.R.; Salamone, Daniel Felipe (2017-03-16)Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains ... -
Efficient edition of the bovine PRNP prion gene in somatic cells and IVF embryos using the CRISPR/Cas9 system
Bevacqua, Romina Jimena; Fernandez-Martín, R.; Savy, Virginia; Canel, Natalia Gabriela; Gismondi, Maria Ines; Kues, Wilfried A.; Carlson, Daniel F.; Fahrenkrug, S.C.; Niemann, H.; Taboga, Oscar Alberto; Ferraris, Sergio; Salamone, Daniel Felipe (2016-11)The recently developed engineered nucleases, such as zinc-finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease ...