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Desarrollo y evaluación de un ELISA de competición para la detección de anticuerpos anti -Brucella en suero y leche de rumiantes
Foster, Camila Nayla; Rossi, Ursula Amaranta; Saracino, María Priscila; Castaño Zubieta, Mirta Raquel; Baldi, Pablo C.; Rossetti, Carlos Alberto (Asociación Argentina de Veterinarios de Laboratorios de Diagnóstico, 2024-09)• El diagnóstico serológico es un insumo clave en los programas de control y erradicación de la brucelosis animal; • Las técnicas serológicas se dividen en pruebas tamiz (o screening) y pruebas complementarias (o ... -
Evaluación preliminar de la polarización fluorescente como prueba DIVA en caprinos vacunados a distintas edades con la cepa Rev.1 de B. melitensis
Rossetti, Carlos Alberto; Foster, Camila Nayla; Olmos, Leandro Hipolito; Rossi, Ursula Amaranta; Castaño Zubieta, Mirta Raquel; Suarez, Victor Humberto (Asociación Argentina de Veterinarios de Laboratorios de Diagnóstico, 2024-09)• La vacunación anti-Brucella está indicada en las cabrillas pre-púberes; • La vacunación de los animales adultos genera anticuerpos persistentes que no se diferencian de los de una infección natural; • La prueba de ... -
Evaluation of B. melitensis whole-cell lysate antigen-based indirect ELISA for the serodiagnosis of caprine brucellosis
Foster, Camila Nayla; Rossi, Ursula Amaranta; Castaño Zubieta, Mirta Raquel; Vanzini, Victor Rene; Rossetti, Carlos Alberto (Elsevier, 2022-10)Different fractions of Brucella (B) abortus or Brucella melitensis have been used as antigens for the detection of anti-Brucella antibodies in goat sera, being their accomplishment cumbersome and time consuming. In an ... -
Subcutaneous vaccination of pregnant guinea pigs with Brucella melitensis Rev.1: a model for preliminary study the safety of vaccine candidates against small ruminant brucellosis
Rossetti, Carlos Alberto; Rossi, Ursula Amaranta; Delgado, Fernando Oscar; Castaño Zubieta, Mirta Raquel; Foster, Camila Nayla (Ediciones INTA, 2024-08)Brucella melitensis is the etiological agent of small ruminant brucellosis and abortion is the only noticeable clinical sign in most cases. Vaccination with the attenuated Rev.1 strain is the best option to prevent this ... -
Validation of an in house iELISA for serodiagnosis of caprine brucellosis and evaluation of the performance of a B. neotomae lysate for the detection of anti-smooth Brucella specific antibodies in ruminants
Foster, Camila Nayla; Rossi, Ursula Amaranta; Rossetti, Carlos Alberto (Elsevier, 2025-03)Enzyme-linked immunosorbent assay (ELISA) is a widely used and effective tool for detection of anti-Brucella antibodies in serum, easy to perform with high sensitivity and specificity. In this study, we validated an in-house ...