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A thermostable laccase from Thermus sp. 2.9 and its potential for delignification of Eucalyptus biomass
Navas, Laura Emilce; Martinez, Fernando; Taverna, María Eugenia; Fetherolf, Morgan M.; Eltis, Lindsay D.; Nicolau, Veronica Viviana; Estenoz, Diana Alejandra; Campos, Eleonora; Benintende, Graciela Beatriz; Berretta, Marcelo Facundo (SpringerOpen, 2019-02)Laccases are multicopper oxidases that are being studied for their potential application in pretreatment strategies of lignocellulosic feedstocks for bioethanol production. Here, we report the expression and characterization ... -
Characterization of a Novel Thermostable Enzyme from Thermus sp. 2.9 with Phospholipase and Acyltransferase Activities
Navas, Laura Emilce; Florin-Christensen, Mónica; Benintende, Graciela Beatriz; Zandomeni, Ruben; Berretta, Marcelo Facundo (Karger, 2018)Phospholipases are classified in different enzyme families according to the ester bond they cleave within phospholipids. The use of phospholipases in industrial processes has prompted the search for new enzymes with ... -
Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
Benintende, Graciela Beatriz; Berretta, Marcelo Facundo; Zandomeni, Ruben; Sauka, Diego Hernan; Amadio, Ariel F.; Ortiz, Elio Maximiliano; Navas, Laura Emilce (2017)We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously ... -
Diversity and distribution of lepidoptera-specific toxin genes in Bacillus thuringiensis strains from Argentina
Sauka, Diego Hernan; Benintende, Graciela Beatriz (2017-05)A total of 268 Bacillus thuringiensis strains obtained from different sources of Argentina were analyzed to determine the diversity and distribution of the cry1, cry2, cry8, cry9 and vip3A genes encoding for lepidopteran-specific ... -
Draft Genome Sequence of Bacillus thuringiensis INTA Fr7-4
Navas, Laura Emilce; Berretta, Marcelo Facundo; Ortiz, Elio Maximiliano; Sauka, Diego Hernan; Benintende, Graciela Beatriz; Zandomeni, Ruben; Amadio, Ariel (2017)We report here the complete annotated 6,035,547-bp draft genome sequence of Bacillus thuringiensis INTA Fr7-4. This strain contains three cry8 and two vip1 and vip2 insecticidal toxin genes. -
Estudio de la toxicidad de proteínas insecticidas de Bacillus thuringiensis como herramienta para optimizar el manejo de lepidópteros plaga de importancia agroeconómica
Onco, Maria Ines (Escuela para Graduados Ing. Agr. Alberto Soriano, Facultad de Agronomía, Universidad de Buenos Aires, 2018-06-26)Entre las plagas principales y recurrentes en las producciones de frutas de pepita se encuentra la “Carpocapsa” (Cydia pomonella Linneaus [Lepidoptera: Tortricidae]). Su control se basa en el empleo de insecticidas químicos ... -
Factores de virulencia de Bacilus thuringiensis y su utilización para el control de coleópteros de alto impacto en el sector agropecuario
Perez, Melisa Paula (Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, 2017-03)La necesidad de contar con herramientas seguras y efectivas para el manejo de plagas, alternativas o complementarias a los insecticidas químicos, estimula el interés en usar entomopatógenos como agentes de biocontrol. ... -
New entomopathogenic strain of Bacillus thuringiensis is able to solubilize different sources of inorganic phosphates
Sauka, Diego Hernan; Piccinetti, Carlos Fabian; Vallejo, Daniela Adriana; Onco, Maria Ines; Perez, Melisa Paula; Benintende, Graciela Beatriz (Elsevier, 2021-04-01)Phosphate-solubilizing strain INTA 293-1 from a soil sample of Corrientes province (Argentina) was identified as Bacillus thuringiensis based on its faculty to produce crystalline inclusions during sporulation and 16S rDNA ... -
Selection of Bacillus thuringiensis strains toxic to cotton boll weevil (Anthonomus grandis, Coleoptera: Curculionidae) larvae = Selección de cepas de Bacillus thuringiensis tóxicas para larvas del picudo del algodonero (Anthonomus grandis, Coleoptera: Curculionidae)
Perez, Melisa Paula; Sauka, Diego Hernan; Onco, Maria Ines; Berretta, Marcelo Facundo; Benintende, Graciela Beatriz (Asociación Argentina de Microbiología, 2017-09)Preliminary bioassays with whole cultures (WC) of 124 Bacillus thuringiensis strains were performed with neonate larvae of Anthonomus grandis, a major cotton pest in Argentina and other regions of the Americas. Three exotic ... -
Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein
Berretta, Marcelo Facundo; Pedarros, Analía Soledad; Sauka, Diego Hernan; Perez, Melisa Paula; Onco, Maria Ines; Benintende, Graciela Beatriz (Elsevier, 2020-05)Bacillus thuringiensis toxins of the Cry1I class have dual specificity for insects in the orders Coleoptera and Lepidoptera. We assessed the toxicity of a Cry1Ia protein from an Argentinian B. thuringiensis strain against ...