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Abstract
Background: Campylobacter fetus is a pathogen of major concern for animal and human health. The species shows a great intraspecific variation, with three subspecies: C. fetus subsp. fetus, C. fetus subsp. venerealis, and C. fetus subsp. testudinum. Campylobacter fetus fetus affects a broad range of hosts and induces abortion in sheep and cows. Campylobacter fetus venerealis is restricted to cattle and causes the endemic disease bovine genital [ver mas...]
dc.contributor.authorIraola, Gregorio
dc.contributor.authorPérez, Ruben
dc.contributor.authorBetancor, Laura
dc.contributor.authorMarandino, Ana
dc.contributor.authorMorsella, Claudia Graciela
dc.contributor.authorMendez, Maria Alejandra
dc.contributor.authorPaolicchi, Fernando
dc.contributor.authorPiccirillo, Alessandra
dc.contributor.authorTomás, Gonzalo
dc.contributor.authorVelilla, Alejandra Vanesa
dc.contributor.authorCalleros, Lucía
dc.date.accessioned2019-03-08T14:37:59Z
dc.date.available2019-03-08T14:37:59Z
dc.date.issued2016-12
dc.identifier.issn1746-6148
dc.identifier.otherhttps://doi.org/10.1186/s12917-016-0913-3
dc.identifier.urihttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-016-0913-3
dc.identifier.urihttp://hdl.handle.net/20.500.12123/4560
dc.description.abstractBackground: Campylobacter fetus is a pathogen of major concern for animal and human health. The species shows a great intraspecific variation, with three subspecies: C. fetus subsp. fetus, C. fetus subsp. venerealis, and C. fetus subsp. testudinum. Campylobacter fetus fetus affects a broad range of hosts and induces abortion in sheep and cows. Campylobacter fetus venerealis is restricted to cattle and causes the endemic disease bovine genital campylobacteriosis, which triggers reproductive problems and is responsible for major economic losses. Campylobacter fetus testudinum has been proposed recently based on genetically divergent strains isolated from reptiles and humans. Both C. fetus fetus and C. fetus testudinum are opportunistic pathogens for immune-compromised humans. Biochemical tests remain as the gold standard for identifying C. fetus but the fastidious growing requirements and the lack of reliability and reproducibility of some biochemical tests motivated the development of molecular diagnostic tools. These methods have been successfully tested on bovine isolates but fail to detect some genetically divergent strains isolated from other hosts. The aim of the present study was to develop a highly specific molecular assay to identify and quantify C. fetus strains. Results: We developed a highly sensitive real-time PCR assay that targets a unique region of the 16S rRNA gene. This assay successfully detected all C. fetus strains, including those that were negative for the cstA gene-based assay used as a standard for molecular C. fetus identification. The assay showed high specificity and absence of cross-reactivity with other bacterial species. The analytical testing of the assay was determined using a standard curve. The assay demonstrated a wide dynamic range between 102 and 107 genome copies per reaction, and a good reproducibility with small intra- and inter-assay variability. Conclusions: The possibility to characterize samples in a rapid, sensitive and reproducible way makes this assay a good option to establish a new standard in molecular identification and quantification of C. fetus species.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherBMCes_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.sourceBMC Veterinary Research 12 : 286 (2016)es_AR
dc.subjectCampylobacter fetuses_AR
dc.subjectPCRes_AR
dc.subjectGenéticaes_AR
dc.subjectGeneticseng
dc.subjectRibosomases_AR
dc.subjectRibosomeseng
dc.subjectExperimentación en Laboratorioes_AR
dc.subjectLaboratory Experimentationeng
dc.subject.otherReacción en Cadena de la Polimerasaes_AR
dc.titleA novel real-time PCR assay for quantitative detection of Campylobacter fetus based on ribosomal sequenceses_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Iraola, Gregorio. Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva; Uruguay. Institut Pasteur Montevideo. Unidad de Bioinformática; Uruguayes_AR
dc.description.filFil: Pérez, Ruben. Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva; Uruguayes_AR
dc.description.filFil: Betancor, Laura. Universidad de la República. Facultad de Medicina. Instituto de Higiene. Departamento de Bacteriología y Virología; Uruguayes_AR
dc.description.filFil: Marandino, Ana. Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva; Uruguayes_AR
dc.description.filFil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Mendez, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Piccirillo, Alessandra. Università degli Studi di Padova. Dipartimento di Biomedicina Comparata e Alimentazione; Italiaes_AR
dc.description.filFil: Tomás, Gonzalo. Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva; Uruguayes_AR
dc.description.filFil: Velilla, Alejandra Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Bacteriología. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentinaes_AR
dc.description.filFil: Calleros, Lucía. Universidad de la República. Facultad de Ciencias. Sección Genética Evolutiva; Uruguayes_AR
dc.subtypecientifico


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