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Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato
Zhao, Xue; Jayarathna, Shishanthi; Turesson, Helle; Fält, Ann Sofie; Nestor, Gustav; Gonzalez, Matías Nicolás; Olsson, Niklas; Beganovic, Mirela; Hofvander, Per; Andersson, Roger; Anderson, Mariette (Nature Publishing Group, 2021-02-22)DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein ... -
Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Springer Netherlands, 2021-01-25)Delivery of the CRISPR/Cas9 components to the plant cells is a key step in its application as a genome editing tool. Here, we compared Agrobacterium-mediated transformation and protoplast transfection with CRISPR/Cas9 ...