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Mostrando ítems 1-5 de 5
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Amylose starch with no detectable branching developed through DNA-free CRISPR-Cas9 mediated mutagenesis of two starch branching enzymes in potato
Zhao, Xue; Jayarathna, Shishanthi; Turesson, Helle; Fält, Ann Sofie; Nestor, Gustav; Gonzalez, Matías Nicolás; Olsson, Niklas; Beganovic, Mirela; Hofvander, Per; Andersson, Roger; Anderson, Mariette (Nature Publishing Group, 2021-02-22)DNA-free genome editing was used to induce mutations in one or two branching enzyme genes (Sbe) in tetraploid potato to develop starch with an increased amylose ratio and elongated amylopectin chains. By using ribonucleoprotein ... -
Comparative potato genome editing: Agrobacterium tumefaciens-mediated transformation and protoplasts transfection delivery of CRISPR/Cas9 components directed to StPPO2 gene
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Anderson, Mariette; Decima Oneto, Cecilia Andrea; Turesson, Helle; Olsson, Niklas; Fält, Ann Sofie; Hofvander, Per; Feingold, Sergio Enrique (Springer Netherlands, 2021-01-25)Delivery of the CRISPR/Cas9 components to the plant cells is a key step in its application as a genome editing tool. Here, we compared Agrobacterium-mediated transformation and protoplast transfection with CRISPR/Cas9 ... -
Edición génica con el sistema CRISPR/Cas9: historia de su descubrimiento y alcances en agricultura
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Feingold, Sergio Enrique (Universidad de Los Andes, Venezuela, 2021-04)La conversión del sistema inmune procariota CRISPR/Cas9 en una herramienta molecular para edición génica representa el avance tecnológico más destacado de la última década, y ha revolucionado tanto la investigación básica ... -
Reduced enzymatic browning in potato tubers by specific editing of a Polyphenol oxidase gene via Ribonucleoprotein complexes delivery of the CRISPR/Cas9 System.
Gonzalez, Matías Nicolás; Massa, Gabriela Alejandra; Andersson, Mariette; Turesson, Helle; Olsson, Niklas; Fält, Ann-Sofie; Storani, Leonardo; Decima Oneto, Cecilia Andrea; Hofvander, Per; Feingold, Sergio Enrique (Frontiers Media, Switzerland, 2020-01-09)Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones, leading to the formation of dark-colored precipitates in fruits and vegetables. This process, known as enzymatic browning, is the cause ... -
Transformación eficiente de Solanum tuberosum cv. Spunta mediada por Agrobacterium tumefaciens utilizando higromicina como agente selectivo
Decima Oneto, Cecilia Andrea; Coronel, Joel; Storani, Leonardo; Gonzalez, Matías Nicolás; Feingold, Sergio Enrique; Massa, Gabriela Alejandra (Ediciones INTA, 2020-08)La transformación genética de plantas es una herramienta biotecnológica de alto impacto en el mejoramiento vegetal que brinda la posibilidad de obtener nuevos cultivos que se adapten a las demandas de producción y de ...