Browsing CICVyA. Centro de Investigación en Ciencias Veterinarias y Agronómicas by Author
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Browsing CICVyA. Centro de Investigación en Ciencias Veterinarias y Agronómicas by Author "Tajima, Hiromi"
Now showing items 1-3 of 3
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Generation of a multi‑herbicide‑tolerant alfalfa by using base editing
Bottero, Ana Emilia; Gomez, Maria Cristina; Stritzler, Margarita; Tajima, Hiromi; Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-02)We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ... -
Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing
Stritzler, Margarita; Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Gomez, Maria Cristina; Frare, Romina Alejandra; Puebla, Andrea Fabiana; Tajima, Hiromi; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ... -
Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
Frare, Romina Alejandra; Stritzler, Margarita; Gomez, Maria Cristina; Tajima, Hiromi; Pascuan, Cecilia Gabriela; López Fernández, María Paula; Bottero, Ana Emilia; Nikel, Pablo I.; Alleva, Karina Edith; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities ...