• Engineering Pseudomonas protegens Pf-5 for nitrogen fixation and its application to improve plant growth under nitrogen-deficient conditions 

      Setten, Lorena; Soto, Gabriela Cinthia; Mozzicafreddo, Matteo; Fox, Ana Romina; Lisi, Christian Daniel; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Diaz Paleo, Antonio Horacio; Ayub, Nicolás Daniel (2013-05-13)
      Nitrogen is the second most critical factor for crop production after water. In this study, the beneficial rhizobacterium Pseudomonas protegens Pf-5 was genetically modified to fix nitrogen using the genes encoding the ...
    • Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1 

      Ayub, Nicolás Daniel; Fox, Ana Romina; Garcia, Araceli Nora; Mozzicafreddo, Matteo; Cuccioloni, Massimiliano; Angeletti, Mauro; Pagano, Elba Maria; Soto, Gabriela Cynthia (Oxford University Press, 2015-01)
      Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. ...
    • Subfunctionalization probably drives the emergence of plant growth‑promoting genes 

      Frare, Romina Alejandra; Mozzicafreddo, Matteo; Gomez, Maria Cristina; Fussoni, Nerina Belen; Soto, Gabriela Cynthia; Ayub, Nicolás Daniel (Springer, 2022-07)
      The duplication and divergence of stress-related genes might help microbes adapt to different environments. However, little is known about this type of subfunctionalization process in microbes. We here examined the evolution ...
    • The Gln15Arg mutation in the transcriptional factor PALM1 produces multifoliate alfalfa 

      Gomez, Maria Cristina; Jozefkowicz, Cintia; Mozzicafreddo, Matteo; Odorizzi, Ariel; Arolfo, Valeria; Basigalup, Daniel Horacio; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-12)
      The CRISPR/Cas9 system has proven to be highly valuable for genome editing in several important crops, including the allogamous tetraploid cultivated alfalfa (Medicago sativa). However, the fact that most of the beneficial ...