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Mostrando ítems 1-16 de 16
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A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
(Springer, 2017-06)LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, ... -
Aspectos epidemiológicos asociados a la Brucelosis Ovina (Brucella ovis) en establecimientos de Tierra del Fuego
(Edición del Autor, 2010-03)La brucelosis ovina, causada por Brucella ovis, es una enfermedad infecciosa que afecta principalmente a los machos, alterando su salud reproductiva. El conocimiento sobre cómo influyen algunos factores en la presencia de ... -
Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
(Scientific Research, 2012-02)Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but ... -
Brucelosis caprina en la Argentina
(Ediciones INTA, 2014)La brucelosis caprina es una enfermedad infecto-contagiosa de distribución mundial, de curso crónico, causada por alguno de los tres biovares de Brucella melitensis, con un alto impacto en la producción caprina, debido a ... -
Clinical Manifestations of Brucellosis in Domestic Animals and Humans
(Bentham Open, 2010)Brucellosis in domestic animals is a chronic disease that is characterized mainly by reproductive signs in cattle, buffaloes, pigs, sheep, goats and dogs. In females the disease is characterized by abortion, placenta ... -
Comparación de la respuesta inmune humoral y celular de las vacunas Brucella abortus cepa RB51 y cepa 19 en vaquillonas Hereford de Patagonia y su interferencia con el diagnóstico serológico de la enfermedad
(Sociedad de Medicina Veterinaria, 2007)Se llevó a cabo un estudio de campo controlado donde se usaron 2 grupos homogéneos de 25 vaquillonas Hereford cada uno, de 10 meses de edad, provenientes de un establecimiento libre de brucelosis y donde nunca se había ... -
Control de la Brucelosis bovina
(EEA Bariloche, INTA, 2021-08)Tras la publicación por parte del SENASA de la resolución 77/2021, se introducen cambios para acelerar y facilitar el control y erradicación de la brucelosis bovina en Argentina -
Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
(Taylor & Francis Group, 2007)Because some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of ... -
Development and evaluation of a species-specific PCR assay for the detection of Brucella ovis infection in rams
(Elsevier, 2010)Brucella ovis infection is a major cause of epididymitis and infertility in rams, resulting in reproductive failure and significant economic losses worldwide. The goal of this study was to develop a PCR test targeting ... -
Estudio de la variabilidad genética de Brucella melitensis en el noroeste de la provincia de San Luis
(Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, 2020)Los objetivos del presente trabajo fueron emplear técnicas de genotipificación molecular en un escenario real, describir genotipos circulantes de Brucella melitensis en el noroeste de la provincia de San Luis y poner a ... -
Evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against Brucella abortus SRB51 in vaccinated heifers
(Elsevier, 2009)The live attenuated Brucella abortus SRB51 (SRB51) is a partial O-chain-deprived mutant. The relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the ... -
A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats
(2017-08)Brucellosis is the leading zoonosis on a worldwide scale and constitutes a major public health threat in many regions of the world. Several molecular markers associated with natural resistance to intracellular bacterial ... -
Molecular characterization of Brucella ovis in Argentina
(Elsevier, 2020-06)Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. ... -
Pathogenesis and immunobiology of brucellosis : review of brucella-host interactions
(Elsevier, 2015-06)This review of Brucellaehost interactions and immunobiology discusses recent discoveries as the basis for pathogenesis-informed rationales to prevent or treat brucellosis. Brucella spp., as animal pathogens, cause human ... -
Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
(Elsevier, 2014-08-15)Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) ... -
Use of Chelex-100 for the molecular diagnosisof five animal pathogens
(Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2021-06)Molecular tools have improved conventional veterinary diagnosis. Acid nucleic extraction is a key step for downstream applications. This work aimed to compare the DNA extraction method Chelex-100 resin (M1) ...