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Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
Reidel, Ivana Gabriela; Garcia, Maria Ines; González, Verónica Doris Guadalupe; Giorello, Antonella; Calvinho, Luis Fernando; Gennaro, Ana Maria; Veaute, Carolina Melania Isabel (International Journal for Research, 2017-06)The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them ... -
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows
Camussone, Cecilia; Reidel, Ivana Gabriela; Molineri, Ana Ines; Cicotello, Joaquin; Miotti, Camila; Suarez Archilla, Guillermo; Curti, C.C.; Veaute, Carolina Melania Isabel; Calvinho, Luis Fernando (Elsevier, 2022-07)The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection ... -
Evaluation of the humoral immune response to a multicomponent recombinant vaccine against S. aureus in healthy pregnant heifers
Pujato, Nazarena; Camussone, Cecilia; Renna, María Sol; Perrig, Melina Soledad; Morein, Bror; Calvinho, Luis Fernando; Marcipar, Iván Sergio (2018-05)Staphylococcus aureus is a worldwide pathogen that causes mastitis in dairy herds. Shortcomings in control programs have encouraged the development of vaccines against this pathogen. This study evaluated the vaccine candidate ... -
Factores de virulencia de Staphylococcus aureus asociados con infecciones mamarias en bovinos: relevancia y rol como agentes inmunógenos = Virulence factors of Staphylococcus aureus associated with intramammary infections in cows: Relevance and role as immunogens
Camussone, Cecilia; Calvinho, Luis Fernando (2013)Staphylococcus aureus es el microorganismo causante de mastitis bovina más prevalente en Argentina y en el mundo. La falta de efectividad frente a este organismo de los métodos tradicionales de control, basados en la higiene ... -
First report of Mycoplasma leachii isolation associated with disease in dairy calves in Argentina = Primer aislamiento de Mycoplasma leachii asociado a enfermedad en terneros de tambo en Argentina
Neder, Veronica Elizabeth; Allassia, Martín; Amadio, Ariel; Calvinho, Luis Fernando (2018)There are few reports about the isolation of Mycoplasma species associated with cattle disease in Argentina. In this work we describe the detection of Mycoplasma leachii associated with disease in dairy calves in Santa Fe ... -
Functional role of antibodies generated in heifers through immunization with Staphylococcus aureus vaccines in invasion and phagocytosis assays
Renna, María Sol; Pereyra, Elizabet Amanda Lorena; Baravalle, Celina; Camussone, Cecilia; Dallard, Bibiana Elisabet; Marcipar, Iván Sergio; Calvinho, Luis Fernando (2014-11)A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised ... -
Genome-wide association study for somatic cell score in Argentinean dairy cattle
Nani, Juan Pablo; Raschia, Maria Agustina; Poli, Mario Andres; Calvinho, Luis Fernando; Amadio, Ariel (2015-05)This study aimed to understand the genomic architecture of Argentinean dairy herds by measuring linkage disequilibrium (LD) and identifying loci associated with parameters calculated from somatic cell count (SCC). Phenotypic ... -
Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
Salimena, Alessandra P.S.; Lange, Carla C.; Camussone, Cecilia; Signorini, Marcelo; Calvinho, Luis Fernando; Brito, María A.V.P.; Borges, Cristiano A.V.; Guimaraes, Alessandro S.; Ribeiro, Joao B.; Mendonca, Leticia C.; Piccoli, Roberta H. (2016-12)Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and ... -
Genotyping and study of the pauA and sua genes of Streptococcus uberis isolates from bovine mastitis = Genotipificación y estudio de los genes pauA y sua de aislamientos de Streptococcus uberis de mastitis bovina
Perrig, Melina Soledad; Ambroggio, Maria Belen; Buzzola, Fernanda R.; Marcipar, Ivan Sergio; Calvinho, Luis Fernando; Veaute, Carolina Melania Isabel; Barbagelata, Maria Sol (2015-12)This study aimed to determine the clonal relationship among 137 Streptococcus uberis isolates from bovine milk with subclinical or clinical mastitis in Argentina and to assess the prevalence and conservation of pauA and ... -
Identificación molecular de especies de mycoplasma de origen bovino aislados de la Cuenca Lechera Central
Neder, Veronica Elizabeth (Facultad de Ciencias Veterinarias de Esperanza, Universidad Nacional del Litoral, 2017)Los Mycoplasma pertenecen a la clase Mollicutes y son los microorganismos procariotas autoreplicables más pequeños que existen en la naturaleza. Estos organismos carecen de pared celular y tienen una limitada capacidad ... -
Immune response and functional role of antibodies raised in heifers against a Staphylococcus aureus CP5 lysate and recombinant antigens vaccine formulated with Iscom Matrix adjuvant
Camussone, Cecilia; Pujato, Nazarena; Renna, María Sol; Veaute, Carolina Melania Isabel; Morein, Bror; Marcipar, Iván Sergio; Calvinho, Luis Fernando (2014-12-15)Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or inactivated ... -
Immune response of heifers against a Staphylococcus aureus CP5 whole cell and lysate vaccine formulated with ISCOM Matrix adjuvant
Camussone, Cecilia; Veaute, Carolina Melania Isabel; Pujato, Nazarena; Morein, Bror; Marcipar, Iván Sergio; Calvinho, Luis Fernando (2014-02)Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells ... -
Immune response of heifers against a Staphylococcus aureus CP5 whole cell vaccine formulated with ISCOMATRIX™ adjuvant
Camussone, Cecilia; Veaute, Carolina Melania Isabel; Porporatto, Carina; Morein, Bror; Marcipar, Iván Sergio; Calvinho, Luis Fernando (2013-02)The shortcomings of Staphylococcus aureus vaccines to control bovine mastitis have been attributed to insufficient capacity of the vaccines to induce opsonizing antibodies and to stimulate cellular immune responses. Types ... -
Immune response of Staphylococcus aureus strains in a mouse mastitis model is linked to adaptive capacity and genotypic profiles
Pereyra, Elizabet Amanda Lorena; Sacco, Sofía Clara; Dure, Andrea; Baravalle, Celina; Renna, María Sol; Andreotti, Carolina Soledad; Monecke, Stefan; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet (2017)Staphylococcus aureus is one of the most frequently isolated major pathogens from intramammary infections (IMI) worldwide. The mechanisms by which S. aureus IMI are established and maintained in dairy cows involve both ... -
Improvement of bovine pestiviral diagnosis by the development of a cost-effective method for detecting viral RNA in fresh specimens and samples spotted in filter papers
Favaro, Paula Melisa; Molineri, Ana Ines; Dus Santos, Maria Jose; Calvinho, Fernando Luis; Pecora, Andrea (Elsevier, 2023-01)Bovine pestiviruses are the causative agents of bovine viral diarrhea, a disease thatcauses severe economic losses in cattle. The aim of this study was to improve their diagnosisby developing a RT-qPCR to detect bovine ... -
In vitro evaluation of ginsenoside Rg1 immunostimulating effect in bovine mononuclear cells
Silvestrini, Paula; Beccaria, Camila; Renna, María Sol; Engler, Carolina; Simonutti, Valeria; Cellone, Ivana; Calvinho, Fernando Luis; Dallard, Bibiana Elisabet; Baravalle, Celina (Elsevier, 2023-05)The aim of this study was to evaluate the immunomodulatory effect of ginsenoside Rg1 on mammary secretion and peripheral blood mononuclear cells (MSMC and PBMC, respectively). The mRNA expression of TLR2, TLR4 and selected ... -
Intramammary infusion of Panax ginseng extract in bovine mammary gland at cessation of milking induces changes in the expression of toll-like receptors, MyD88 and NF-kB during early involution
Baravalle, Celina; Silvestrini, Paula; Cadoche, Mónica C.; Beccaria, Camila; Andreotti, Carolina Soledad; Renna, Maria Sol; Pereyra, Elizabet Amanda Lorena; Ortega, Hugo Hector; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet (2015-06)The purposes of this study were to explore TLR2 and TLR4 participation and MyD88 and NF-κB activation in bovine mammary glands (BMG) treated with Panax ginseng (PG) at involution and verify the effect of PG in the cytokine ... -
Intramammary infusion of Panax ginseng extract in the bovine mammary gland at cessation of milking modifies components of the insulin-like growth factor system during involution
Dallard, Bibiana Elisabet; Pujato, Silvina A.; Baravalle, Celina; Pereyra, Elizabet Amanda Lorena; Rey, Florencia; Renna, Maria Sol; Calvinho, Luis Fernando (2013-06)The objective of this study was to evaluate the effects of a single intramammary infusion of Panax ginseng extract (GS) on insulin-like growth factors (IGF) in bovine mammary gland during early involution. Eight mammary ... -
Intramammary inoculation of Panax ginseng plays an immunoprotective role in Staphylococcus aureus infection in a murine model
Silvestrini, Paula; Beccaria, Camila; Pereyra, Elizabet Amanda Lorena; Renna, María Sol; Ortega, Hugo Hector; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet; Baravalle, Celina (2017-12)The immunoprotective effect of Panax ginseng (Pg) extract was investigated in a mouse mastitis model. Lactating female mice were intramammarily inoculated with Pg or placebo, and then were challenged with S. aureus, while ... -
Involvement of matrix metalloproteinases and their inhibitors in Staphylococcus aureus chronically infected bovine mammary glands during active involution
Beccaria, Camila; Velázquez, Natalia S.; Chervaz, Victoria; Pirola, Silvana I.; Baravalle, Celina; Renna, María Sol; Calvinho, Luis Fernando; Dallard, Bibiana Elisabet (Elsevier, 2021-04)The aim of this study was to characterize the protein expression of matrix metalloproteinase-2 (MMP-2) and ‐− 9 and their inhibitors (TIMP-1 and -2) in mammary tissue of dairy cows with naturally occurring chronic S. aureus ...