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    • CRISPR/Cas9-mediated knockout of a polyester synthase-like gene delays flowering time in alfalfa 

      Galindo Sotomonte, Luisa Fernanda; Jozefkowicz, Cintia; Gomez, Maria Cristina; Stritzler, Margarita; Frare, Romina Alejandra; Bottero, Ana Emilia; Tajima, Hiromi; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2023-02)
      Alfalfa (Medicago sativa), commonly known as the “Queen of Forages”, is the main source of plant protein for meat and milk production systems worldwide. Since alfalfa is a perennial vegetative-harvest forage, delaying ...

    • CRISPR/Cas9‑mediated knockout of SPL13 radically increases lettuce yield 

      Beracochea, Valeria Cecilia; Stritzler, Margarita; Radonic, Laura Mabel; Bottero, Ana Emilia; Jozefkowicz, Cintia; Darqui, Flavia Soledad; Ayub, Nicolás Daniel; Lopez Bilbao, Marisa Gisela; Soto, Gabriela Cynthia (Springer, 2022-12)
      Cultivated lettuce (Lactuca sativa L.) is a diploid (2n = 2x = 18), autogamous species. This popular leafy vegetable is considered one of the first crops (Guo et al. 2022). Currently, lettuce is the third most consumed ...

    • Efficient CRISPR/Cas9 genome editing in alfalfa using a public germplasm 

      Bottero, Ana Emilia; Massa, Gabriela Alejandra; Gonzalez, Matías Nicolás; Stritzler, Margarita; Tajima, Hiromi; Gomez, Maria Cristina; Frare, Romina Alejandra; Feingold, Sergio Enrique; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Frontiers Media, 2021-07)
      Because its ability to acquire large amounts of nitrogen by symbiosis, tetraploid alfalfa is the main source of vegetable proteins in meat and milk production systems in temperate regions. Alfalfa cultivation also adds ...

    • Generation of a multi‑herbicide‑tolerant alfalfa by using base editing 

      Bottero, Ana Emilia; Gomez, Maria Cristina; Stritzler, Margarita; Tajima, Hiromi; Frare, Romina Alejandra; Pascuan, Cecilia Gabriela; Blumwald, Eduardo; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2022-02)
      We present the first report on base editing in alfalfa. Specifically, we showed edited alfalfa with tolerance to both sulfonylurea- and imidazolinone-type herbicides. Alfalfa (Medicago sativa) is one of the most important ...

    • Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR 

      Bottero, Ana Emilia; Pascuan, Cecilia Gabriela; Pagano, Elba Maria; Ayub, Nicolás Daniel; Soto, Gabriela Cinthia; Jozefkowicz, Cintia (2016)
      Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the ...

    • pBAR–H3.2, a native-optimized binary vector to bypass transgene silencing in alfalfa 

      Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Kapros, Tamas; Ayub, Nicolás Daniel; Soto, Gabriela Cynthia (Springer, 2020-03)
      Alfalfa is the main forage worldwide due to its high biomass production, excellent nutritional qualities and adaptation to a wide range of environments (Singer et al. 2018). Besides, due to its ability to grow without ...

    • Rapid and cloning‑free screening of edited alfalfa via next‑generation sequencing 

      Stritzler, Margarita; Pascuan, Cecilia Gabriela; Bottero, Ana Emilia; Gomez, Maria Cristina; Frare, Romina Alejandra; Puebla, Andrea Fabiana; Tajima, Hiromi; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The identification of full knockout plants is frequently the rate-limiting step of the research and commercial projects searching the complete loss of the function of target genes via the CRISPR/Cas9 system. This problem ...

    • Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis 

      Frare, Romina Alejandra; Stritzler, Margarita; Gomez, Maria Cristina; Tajima, Hiromi; Pascuan, Cecilia Gabriela; López Fernández, María Paula; Bottero, Ana Emilia; Nikel, Pablo I.; Alleva, Karina Edith; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia (Springer, 2022-07)
      The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities ...