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Analysis of sequence variations in the GABA gated chloride gene associated with resistance to fipronil in Rhipicephalus (Boophilus) microplus ticks from Argentina
Sarli, Macarena; Torrents, Jorgelina; Morel, Nicolas; Nava, Santiago (Elsevier, 2022-11)This study aimed to evaluate the sequence variations of the GABA-Cl gene of Rhipicephalus (Boophilus) microplus ticks from Argentina with different resistance levels to fipronil. Genomic DNA was obtained from engorged ... -
Casos de anaplasmosis, babesiosis y tripanosomiasis bovina registradas en el INTA Rafaela (2012-2019)
Mazzucco Panizza, Matilde Nahimé; Novoa, María Belén; Sarli, Macarena; Signorini Porchiett, Marcelo Lisandro; Echaide, Ignacio Eduardo (Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste, 2022-06)Anaplasmosis, babesiosis y tripanosomiasis de los bovinos son enfermedades de importancia económica provocadas por Anaplasma marginale, Babesia bovis y/o Babesia bigemina, y Trypanosoma vivax, respectivamente. En este ... -
Development and evaluation of a double-antigen sandwich ELISA to identify Anaplasma marginale–infected and A. centrale–vaccinated cattle
Sarli, Macarena; Thompson, Carolina Soledad; Novoa, María Belen; Valentini, Beatriz Susana; Mastropaolo, Mariano; Echaide, Ignacio Eduardo; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2019-11)Bovine anaplasmosis is a worldwide infectious disease caused by the intraerythrocytic bacterium Anaplasma marginale, which is transmitted by ticks and fomites. A. centrale is a less virulent subspecies used as a live ... -
Development of a novel fusion protein with Anaplasma marginale and A. centrale MSP5 improved performance of Anaplasma antibody detection by cELISA in infected and vaccinated cattle
Primo, María Evangelina; Thompson, Carolina Soledad; Valentini, Beatriz Susana; Sarli, Macarena; Novoa, María Belen; Mangold, Atilio Jose; Torioni, Susana Marta (Plos One, 2019-01-23)Detection of antibodies to Anaplasma spp. using commercial competitive enzyme-linked immunosorbent assay (ccELISA) is based on the recombinant major surface protein 5 fused to maltose binding protein (MBP-MSP5) or glutathione ... -
Influence of the cattle body weight estimation for drug dosing on the pharmacokinetic and efficacy of ivermectin against Rhipicephalus (Boophilus) microplus
Miro, Maria Victoria; Sarli, Macarena; Rossner, Maria Victoria; Gomez, Viviana Daniela; Toffaletti, Jose Rodolfo; Lifschitz, Adrian Luis; Nava, Santiago (Systematic and Applied Acarology Society, 2022-08)This study aimed to evaluate how the method of body weight estimation of a herd (average vs individual) for drug dosing may affect the pharmacokinetics and therapeutic efficacy of ivermectin 3.15% against Rhipicephalus ... -
Neospora caninum truncated recombinant proteins formulated with liposomes and CpG-ODNs triggered a humoral immune response in cattle after immunisation and challenge
Novoa, María Belén; Sarli, Macarena; Reidel, Ivana Gabriela; Veaute, Carolina Melania Isabel; Valentini, Beatriz Susana; Primo, María Evangelina (Elsevier, 2021-06)Abortions caused by Neospora caninum are a serious problem in cattle production and require effective immunoprophylaxis. The objective of this work was to assess the humoral immune response to four recombinant (r) N. caninum ... -
Producción y evaluación de antígenos recombinantes para el diagnóstico serológico y la generación de vacunas para el control de la anaplasmosis bovina
Sarli, Macarena (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2020)La anaplasmosis bovina, causada por Anaplasma marginale (rickettsia intraeritrocítica transmitida por artrópodos hematófagos), es una enfermedad infecciosa de gran interés por las severas pérdidas económicas que ocasiona. ... -
Producción y evaluación de antígenos recombinantes para el diagnóstico serológico y la generación de vacunas para el control de la anaplasmosis bovina
Sarli, Macarena (Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, 2020)En este trabajo se abordó la anaplasmosis de forma integral, desde el diagnóstico y la prevención. La evaluación serológica de la enfermedad se realiza por ELISA de competición (ELISAc), utilizando como antígeno a la ... -
Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fluazuron in Argentina
Torrents, Jorgelina; Sarli, Macarena; Sarmiento, Nestor Fabian; Rossner, Maria Victoria; Morel, Nicolas; Guglielmone, Alberto; Nava, Santiago (Springer, 2022-05)The aim of this work is to report the presence of resistance to fluazuron in a population of Rhipicephalus microplus in Argentina. The evidence was obtained from field and in vitro trials. In the field trial, cattle infested ... -
Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina
Torrents, Jorgelina; Sarli, Macarena; Rossner, Maria Victoria; Toffaletti, Jose Rodolfo; Morel, Nicolas; Martinez, Norberto Claudio; Webster, Anelise; Mangold, Atilio Jose; Guglielmone, Alberto; Nava, Santiago (Elsevier, 2020)Resistance to ivermectin in populations of the cattle tick Rhipicephalus microplus in Argentina was diagnosed in this work. The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of ... -
Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
Primo, María Evangelina; Sarli, Macarena; Thompson, Carolina Soledad; Torioni, Susana Marta; Echaide, Ignacio Eduardo (Elsevier, 2021-09)The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem ... -
Successive treatments with ivermectin (3.15%) to control the tick Rhipicephalus (Boophilus) microplus in cattle: Pharmacokinetic and efficacy assessment
Sarli, Macarena; Miró, Maria Victoria; Rossner, Maria Victoria; Nava, Santiago; Lifschitz, Adrian Luis (Elsevier, 2022-01)This study aimed to evaluate the pharmacokinetics, the potential accumulation in the body of treated animals and the efficacy of ivermectin long-acting formulation (3.15%) against the cattle tick Rhipicephalus (Boophilus) ... -
A vaccine using Anaplasma marginale subdominant type IV secretion system recombinant proteins was not protective against a virulent challenge
Sarli, Macarena; Novoa, María Belen; Mazzucco, Matilde N.; Signorini, Marcelo; Echaide, Ignacio Eduardo; Echaide, Susana T. de; Primo, María Evangelina (PLOS; National Center for Biotechnology Information, 2020-02)Anaplasma marginale is the most prevalent tick-borne livestock pathogen with worldwide distribution. Bovine anaplasmosis is a significant threat to cattle industry. Anaplasmosis outbreaks in endemic areas are prevented via ... -
Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
Novoa, María Belen; Aguirre, Nerina; Ormaechea, Nadia; Palmero, Sebastián; Rouzic, Lisandro; Valentini, Beatriz Susana; Sarli, Macarena; Orcellet, Viviana Mercedes; Marengo, Rafael; Vanzini, Victor Rene; Primo, María Evangelina (Elsevier, 2020-08)Neospora caninum is a protozoan parasite that causes abortion and reproductive failure in small ruminants. We validated and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein ...