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Evaluation of PMA‐qPCR methodology to detect and quantify viable Shiga toxin‐producing Escherichia coli in beef burgers Rey, Maria De Los Angeles; Cap, Mariana; Favre, Leonardo Cristian; Rodriguez, Anabel; Dus Santos, Maria Jose; Vaudagna, Sergio Ramon; Mozgovoj, Marina Valeria (2021-02-06)Propidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real‐time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA‐qPCR ...
High-pressure processing treatment of beef burgers: Effect on Escherichia coli O157 inactivation evaluated by plate count and PMA-qPCR Rey, Maria De Los Angeles; Rodriguez, Anabel; Rossi Ribeiro, Luma; Dos Santos Cruz, Fabiano; Cap, Mariana; Mozgovoj, Marina Valeria; Cristianini, Marcelo; Vaudagna, Sergio Ramon (Wiley, 2022-04-20)Propidium monoazide coupled to real time PCR (PMA-qPCR) is a novel methodology proposed for the quantification of viable bacteria in food after microbial inactivation treatments. The aim of this work was to assess the ...