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A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1
Kornuta, Claudia Alejandra; Langellotti, Cecilia Ana; Bidart, , Juan Esteban; Soria, Ivana; Quattrocchi, Valeria; Gammella, Mariela; Cheuquepan Valenzuela, Felipe; Mignaqui, Ana Clara; Ferraris, Sergio; Charleston, Bryan; Hecker, Yanina; Moore, Prando Dadin; Zamorano, Patricia Ines (Elsevier, 2021-02)DNA vaccines are capable of inducing humoral and cellular immunity, and are important to control bovine herpesvirus 1 (BoHV-1), an agent of the bovine respiratory disease complex. In previous work, a DNA plasmid that encodes ... -
Advances in novel vaccines for foot and mouth disease: focus on recombinant empty capsids
Mignaqui, Ana Clara; Ruiz, Vanesa; Durocher, Yves; Wigdorovitz, Andres (Taylor & Francis, 2019-05)Foot and mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals, which causes severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). ... -
Bovine dendritic cell activation, T cell proliferation and antibody responses to foot-and-mouth disease, is similar with inactivated virus and virus like particles
Quattrocchi, Valeria; Bidart, Juan Esteban; Mignaqui, Ana Clara; Ruiz, Vanesa; Ferella, Alejandra; Langellotti, Cecilia Ana; Gammella, Mariela; Ferraris, Sergio; Carrillo, Jorge Ernesto; Wigdorovitz, Andres; Durocher, Yves; Cardillo, Sabrina Beatriz; Charleston, Bryan; Zamorano, Patricia Ines (Frontiers Media, 2020-09)Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals that causes severe economic losses in the livestock industry. Currently available vaccines are based on the inactivated FMD virus (FMDV). ... -
Comparison of strategies for the production of FMDV empty capsids using the Baculovirus Vector System
Ruiz, Vanesa; Mignaqui, Ana Clara; Nuñez, M.C.; Reytor, Edel; Escribano, José M.; Wigdorovitz, Andres (Springer, 2014-11)Recombinant FMDV empty capsids have been produced in insect cells and larvae using the baculovirus expression system, although protein yield and efficiency of capsid assembly have been highly variable. In this work, two ... -
Comparison of transient and stable expression of foot-and-mouth disease virus capsid proteins in mammalian cells
Mignaqui, Ana Clara; Ruiz, Vanesa; Wigdorovitz, Andres (Scientific Research, 2013-12)Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant ... -
Development of a duplex PCR for the identification of Fasciola hepatica in lymnaeid snails
Mignaqui, Ana Clara; Alvarez, Lucia Paula; Soler, Paula; Larroza, Marcela Patricia (Ediciones INTA, 2020-12)Fasciola hepatica is a parasitic trematode that causes fascioliasis, a disease that affects domestic livestock and humans. The complex life cycle of F. hepatica involves lymnaeid snails as intermediate hosts. Detection of ... -
Enteritis hemorrágica en un equino atribuida a Clostridium perfringens tipo A.
Robles, Carlos Alejandro; Paván, María Elisa; Chapero, Claudio; Marcellino, Romanela; Cairó, Fabián A.; Mignaqui, Ana Clara (Veterinaria Argentina S.R.L., 2016-06)La muerte de equinos con cuadros clínicos de cólico es frecuente, especialmente en animales estabulados y con fines deportivos. Sin embargo, al ser muy bajo el porcentaje de animales que son necropsiados y muestreados, la ... -
FMD empty capsids combined with the immunostant particle adjuvant -ISPA or ISA206 induce protective immunity against foot and mouth disease virus
Bidart, Juan Esteban; Mignaqui, Ana Clara; Kornuta, Claudia Alejandra; Lupi, Giuliana; Gammella, Mariela; Soria, Ivana; Galarza, Roxana Ivon; Ferella, Alejandra; Cardillo, Sabrina Beatriz; Langellotti, Cecilia Ana; Quattrocchi, Valeria; Durocher, Yves; Wigdorovitz, Andres; Marcipar, Ivan; Zamorano, Patricia Ines (Elsevier, 2021-05)Foot and Mouth Disease Virus (FMDV) causes economy losses and is controlled by vaccination in many countries. Vaccine formulations based on empty capsids or Virus-Like Particles (VLPs) have the advantage of avoiding the ... -
Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine
Mignaqui, Ana Clara; Ferella, Alejandra; Cass, Brian; Mukankurayija, Larissa; L’Abbé, Denis; Bisson, Louis; Sánchez, Cintia; Scian, Romina; Cardillo, Sabrina Beatriz; Durocher, Yves; Wigdorovitz, Andres (Frontiers Media S.A., 2020-09)Inactivated Foot-and-Mouth Disease (FMD) vaccine has proven to be effective in the control of the disease. However, its production has some disadvantages, including the costly biosafety facilities required for the production ... -
Hacia una vacuna contra el Coronavirus causante de la Covid-19
Mignaqui, Ana Clara; Ghersa, Federica; Marcellino, Romanela; Faraldo, Joaquin; Carignano, María de los Angeles; Tatsch, Veronica; De la Puente, Eduardo; Pappalardo, Juan Sebastián (EEA Bariloche, INTA, 2021-08)En el Grupo de Nanomedicina Veterinaria del IFAB (INTA EEA Bariloche/CONICET), junto a la Dirección Médica de Laboratorios Bagó S.A., estamos trabajando en el desarrollo de una vacuna génica contra SARS-CoV-2, económica, ... -
Isolation and molecular characterization of Clostridium perfringens from healthy Merino lambs in Patagonia region, Argentina
Mignaqui, Ana Clara; Marcellino, Romanela; Pappalardo, Juan Sebastián; Robles, Carlos Alejandro; Ronco, T.; Nonnemann, B.; Pedersen, K. (2016)The presence and molecular characterization of Clostridium perfringens in healthy Merino lambs over a six-month period was investigated in this study. Overall, a high prevalence of C. perfringens was detected, even in ... -
Procesamiento del material caseoso para determinar la presencia de C. Pseudotuberculosis en ovinos
Marcellino, Romanela Beatriz; Raineri, Mariana; Mignaqui, Ana Clara; Santana, Jorge Luis (Sociedad de Medicina Veterinaria (Argentina), 2017-11)La Linfoadenitis Caseosa (LAC), es una enfermedad infectocontagiosa crónica causada por Corynebacterium pseudotuberculosis que se caracteriza por la formación de abscesos en ganglios y órganos. En la Patagonia, es común ... -
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
Mignaqui, Ana Clara; Ruiz, Vanesa; Perret, Sylvie; St-Laurent, Gilles; Chahal, Parminder Singh; Transfiguracion, Julia; Sammarruco, Romina Ayelén; Gnazzo, Victoria; Durocher, Yves; Wigdorovitz, Andres (PLOS, 2013-08-20)Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). ...