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Advances in novel vaccines for foot and mouth disease: focus on recombinant empty capsids
Mignaqui, Ana Clara; Ruiz, Vanesa; Durocher, Yves; Wigdorovitz, Andres (Taylor & Francis, 2019-05)Foot and mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals, which causes severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). ... -
Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
Pecora, Andrea; Perez Lopez, Jorgelina; Jordan, Maximiliano Jesus; Franco, Lautaro Nahuel; Politzki, Romina Paula; Ruiz, Vanesa; Alvarez, Irene (Sage Publications, 2020-11)Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study ... -
BLV: lessons on vaccine development
Abdala, Alejandro Ariel; Alvarez, Irene; Brossel, Hélène; Calvinho, Luis Fernando; Carignano, Hugo; Franco, Lautaro Nahuel; Gazon, Hélène; Gillissen, Christelle; Hamaidia, Malik; Hoyos, Clotilde; Jacques, Jean-Rock; Joris, Thomas; Laval, Florent; Petersen, Marcos Iván; Porquet, Florent; Porta, Natalia Gabriela; Ruiz, Vanesa; Safari, Rogaiyeh; Suarez Archilla, Guillermo Alejandro; Trono, Karina Gabriela; Willems, Luc (BMC, 2019-10-07)Vaccination against retroviruses is a challenge because of their ability to stably integrate into the host genome, undergo long-term latency in a proportion of infected cells and thereby escape immune response. Since ... -
Bovine dendritic cell activation, T cell proliferation and antibody responses to foot-and-mouth disease, is similar with inactivated virus and virus like particles
Quattrocchi, Valeria; Bidart, Juan Esteban; Mignaqui, Ana Clara; Ruiz, Vanesa; Ferella, Alejandra; Langellotti, Cecilia Ana; Gammella, Mariela; Ferraris, Sergio; Carrillo, Jorge Ernesto; Wigdorovitz, Andres; Durocher, Yves; Cardillo, Sabrina Beatriz; Charleston, Bryan; Zamorano, Patricia Ines (Frontiers Media, 2020-09)Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals that causes severe economic losses in the livestock industry. Currently available vaccines are based on the inactivated FMD virus (FMDV). ... -
Bovine leukemia virus infection in neonatal calves. Risk factors and control measures
Ruiz, Vanesa; Porta, Natalia Gabriela; Lomonaco, Marina; Trono, Karina Gabriela; Alvarez, Irene (Frontiers Media, 2018-10)Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). Although efficient eradication programs have been successfully implemented in most European countries and Oceania, BLV infection rates ... -
Comparison of strategies for the production of FMDV empty capsids using the Baculovirus Vector System
Ruiz, Vanesa; Mignaqui, Ana Clara; Nuñez, M.C.; Reytor, Edel; Escribano, José M.; Wigdorovitz, Andres (Springer, 2014-11)Recombinant FMDV empty capsids have been produced in insect cells and larvae using the baculovirus expression system, although protein yield and efficiency of capsid assembly have been highly variable. In this work, two ... -
Comparison of transient and stable expression of foot-and-mouth disease virus capsid proteins in mammalian cells
Mignaqui, Ana Clara; Ruiz, Vanesa; Wigdorovitz, Andres (Scientific Research, 2013-12)Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant ... -
Experimental infection of sheep with bovine leukemia virus (BLV): minimum dose of BLV-FLK cells and cell-free BLV and neutralization activity of natural antibodies = Infección experimental en ovinos con el virus de leucosis bovina: dosis mínima de células BLV-FLK y virus libre de células, y actividad neutralizante de anticuerpos naturales
Porta, Natalia Gabriela; Alvarez, Irene; Suarez Archilla, Guillermo Alejandro; Ruiz, Vanesa; Abdala, Alejandro Ariel; Trono, Karina Gabriela (Elsevier España, 2019-12)Bovine leukemia virus (BLV) is an important cattle pathogen that causes major economic losses worldwide, especially in dairy farms. The use of animal models provides valuable insight into the pathogenesis of viral infections. ... -
Incidencia de la garrapata común del bovino en la transmisión del virus de la leucosis bovina
Martinez, Norberto Claudio; Nava, Santiago; Ruiz, Vanesa (Ediciones INTA; Estación Experimental Agropecuaria Reconquista, 2021-01)Este trabajo tiene como finalidad, determinar el rol de la larva de la garrapata común del bovino (Rhipicephalus microplus) en la transmisión del Virus de la Leucosis Bovina (VLB) de un animal enfermo a otro susceptible. ... -
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
Ruiz, Vanesa; Baztarrica, Josefina; Rybicki, Edward P.; Meyers, Ann E.; Wigdorovitz, Andres (Elsevier, 2018-12)Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The ... -
Plant‐produced viral bovine vaccines: what happened during the last 10 years?
Ruiz, Vanesa; Mozgovoj, Marina Valeria; Dus Santos, Maria Jose; Wigdorovitz, Andres (Wiley, 2015-10)Vaccination has proved to be an efficient strategy to deal with viral infections in both human and animal species. However, protection of cattle against viral infections is still a major concern in veterinary science. ... -
Seroprevalencia de leucosis enzoótica bovina en tambos del sur de la provincia de Córdoba, Argentina = Seroprevalence of enzootic bovine leucosis in dairy farms in the south of the province of Córdoba, Argentina
Mació, M.; Magnano, Gabriel; Porta, Natalia Gabriela; Petersen, Marcos Iván; Macias, Analía; Sticotti, E.; Ruiz, Vanesa; Schneider, M.; Giraudo, J. (2019-03)La Leucosis Enzoótica Bovina (LEB) afecta al tejido linfoide y el rasgo patológico distintivo es la transformación neoplásica de linfocitos B, su frecuencia es predominante en el ganado lechero adulto. Existen pocos estudios ... -
Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids
Mignaqui, Ana Clara; Ruiz, Vanesa; Perret, Sylvie; St-Laurent, Gilles; Chahal, Parminder Singh; Transfiguracion, Julia; Sammarruco, Romina Ayelén; Gnazzo, Victoria; Durocher, Yves; Wigdorovitz, Andres (PLOS, 2013-08-20)Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). ...