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An Ibero-American inter-laboratory trial to evaluate serological tests for the detection of anti-Neospora caninum antibodies in cattle
Campero, Lucía María; Moreno-Gonzalo, Javier; Venturini, María Cecilia; Moré, Gastón; Dellarupe, Andrea; Rambeaud, Magdalena; Echaide, Ignacio Eduardo; Valentini, Beatriz Susana; Campero, Carlos Manuel; Moore, Prando Dadin; Cano, Dora Beatriz; Fort, Marcelo Cristian; Mota, Rinaldo A.; Serrano-Martínez, Marcos E.; Cruz-Vázquez, Carlos; Ortega-Mora, Luis M.; Álvarez-García, Gema (2018-01)We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories ... -
Babesia bovis biological clones and the inter-strain allelic diversity of the Bv80 gene support subpopulation selection as a mechanism involved in the attenuation of two virulent isolates
Baravalle, María Eugenia; Thompson, Carolina Soledad; Valentini, Beatriz Susana; Ferreira, Mariano B.; Torioni, Susana Marta; Florin-Christensen, Mónica; Echaide, Ignacio Eduardo (Elsevier, 2012-12-21)The virulence phenotype of Babesia bovis subpopulations was evaluated using biological clones derived from the high-virulence BboS2P and the low-virulence BboR1A strain and two original virulent isolates, BboL15 and BboL17, ... -
Development and evaluation of a double-antigen sandwich ELISA to identify Anaplasma marginale–infected and A. centrale–vaccinated cattle
Sarli, Macarena; Thompson, Carolina Soledad; Novoa, María Belen; Valentini, Beatriz Susana; Mastropaolo, Mariano; Echaide, Ignacio Eduardo; Torioni, Susana Marta; Primo, María Evangelina (American Association of Veterinary Laboratory Diagnosticians, 2019-11)Bovine anaplasmosis is a worldwide infectious disease caused by the intraerythrocytic bacterium Anaplasma marginale, which is transmitted by ticks and fomites. A. centrale is a less virulent subspecies used as a live ... -
Development and field evaluation of a nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) analysis to identify A. marginale-infected and A. centrale-vaccinated cattle
Primo, María Evangelina; Bellezze, Julio; Morel, Nicolas; Mazzucco Panizza, Matilde Nahimé; Valentini, Beatriz Susana; Torioni, Susana Marta; Thompson, Carolina Soledad (Elsevier, 2022-07)A nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) assay based on the amplification of the Anaplasma spp. highly conserved msp5 gene and posterior digestion with HindIII endonuclease ... -
Development of a novel fusion protein with Anaplasma marginale and A. centrale MSP5 improved performance of Anaplasma antibody detection by cELISA in infected and vaccinated cattle
Primo, María Evangelina; Thompson, Carolina Soledad; Valentini, Beatriz Susana; Sarli, Macarena; Novoa, María Belen; Mangold, Atilio Jose; Torioni, Susana Marta (Plos One, 2019-01-23)Detection of antibodies to Anaplasma spp. using commercial competitive enzyme-linked immunosorbent assay (ccELISA) is based on the recombinant major surface protein 5 fused to maltose binding protein (MBP-MSP5) or glutathione ... -
Development, validation and field evaluation of an indirect ELISA for the detection of antibodies against Brucella abortus in bulk and individual milk samples in dairy cattle
Novoa, María Belén; Aguirre, Nerina; Valentini, Beatriz Susana; Torioni, Susana Marta; Signorini Porchiett, Marcelo Lisandro; Primo, María Evangelina; Elena, Sebastián Alejandro; Vanzini, Victor Rene (Elsevier, 2022-11)Brucellosis is an abortigenic and zoonotic disease. In cattle, it is mainly caused by Brucella abortus. The disease is endemic in low- and middle-income countries, being considered a neglected zoonotic disease. In these ... -
Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle
Novoa, María Belén; Valentini, Beatriz Susana; Torioni, Susana Marta; Primo, María Evangelina; Echaide, Ignacio Eduardo (American Association of Veterinary Laboratory Diagnosticians (AAVLD), 2020-05)Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. There are no treatments or vaccines available; disease control is based on diagnosis and herd management ... -
Immunisation of cattle against Babesia bovis combining a multi-epitope modified vaccinia Ankara virus and a recombinant protein induce strong Th1 cell responses but fails to trigger neutralising antibodies required for protection
Jaramillo Ortiz, Jose Manuel; Paoletta, Martina; Gravisaco, María José; Lopez Arias, Ludmila Sol; Montenegro, Valeria Noely; De La Fourniere, Sofia; Valenzano, Magali Nicole; Guillemi, Eliana Carolina; Valentini, Beatriz Susana; Echaide, Ignacio Eduardo; Farber, Marisa Diana; Wilkowsky, Silvina Elizabeth (Elsevier, 2019-10)Protection against the intraerythrocytic protozoan parasite Babesia bovis depends on both strong innate and adaptive immune response, this latter involving the presentation of parasite antigens to CD4+ T-lymphocytes by ... -
Neospora caninum truncated recombinant proteins formulated with liposomes and CpG-ODNs triggered a humoral immune response in cattle after immunisation and challenge
Novoa, María Belén; Sarli, Macarena; Reidel, Ivana Gabriela; Veaute, Carolina Melania Isabel; Valentini, Beatriz Susana; Primo, María Evangelina (Elsevier, 2021-06)Abortions caused by Neospora caninum are a serious problem in cattle production and require effective immunoprophylaxis. The objective of this work was to assess the humoral immune response to four recombinant (r) N. caninum ... -
Typification of virulent and low virulence Babesia bigemina clones by 18S rRNA and rap-1c
Thompson, Carolina Soledad; Baravalle, María Eugenia; Valentini, Beatriz Susana; Mangold, Atilio Jose; Torioni, Susana Marta; Ruybal, Paula; Farber, Marisa Diana; Echaide, Ignacio Eduardo (2014-06)The population structure of original Babesia bigemina isolates and reference strains with a defined phenotypic profile was assessed using 18S rRNA and rap-1c genes. Two reference strains, BbiS2P-c (virulent) and BbiS1A-c ... -
Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus)
Novoa, María Belén; Soler, Juan Pablo; Cirone, Karina Mariela; Hecker, Yanina; Valentini, Beatriz Susana; Primo, María Evangelina; Moore, Prando Dadin (Elsevier, 2023-01)Neospora caninum is a protozoan parasite that cause abortion in different ruminant species, including red deer ( Cervus elaphus). There are no validated assays to be performed with sera from red deer. At the present work, ... -
Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
Novoa, María Belen; Aguirre, Nerina; Ormaechea, Nadia; Palmero, Sebastián; Rouzic, Lisandro; Valentini, Beatriz Susana; Sarli, Macarena; Orcellet, Viviana Mercedes; Marengo, Rafael; Vanzini, Victor Rene; Primo, María Evangelina (Elsevier, 2020-08)Neospora caninum is a protozoan parasite that causes abortion and reproductive failure in small ruminants. We validated and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein ...