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Aislamiento y caracterización de regiones promotoras de girasol para dirigir la expresión tejido específica de genes foráneos a semilla
Zavallo, Diego (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2014-04)Desde el inicio de la utilización de variedades transgénicas, la superficie agrobiotecnológica ha aumentado exponencialmente, con un incremento del orden de 80 veces en solo 14 años, y su uso se ha expandido a más de veinte ... -
Biotechnological improvement of ornamental plants
Darqui, Flavia Soledad; Radonic, Laura Mabel; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (2017)The discovery of commercial transgenic varieties of orange petunias sold in Europe and the United States although they had never reached the approved status, and the consequent recommendation to destroy them, was the trigger ... -
Nuevas estrategias para la transformación y expresión de genes de interés en girasol
Radonic, Laura Mabel (Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2011-09)El girasol es una especie de la familia Asteraceae (Compositae) de gran importancia económica, considerada hasta hace una década recalcitrante al cultivo in vitro y la transformación genética. En un trabajo previo (Radonic, ... -
Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce
Darqui, Flavia Soledad; Radonic, Laura Mabel; Beracochea, Valeria Cecilia; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (Frontiers Publishing, 2021-11)The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated ... -
Potato snakin-1 gene enhances tolerance to Rhizoctonia solani and Sclerotinia sclerotiorum in transgenic lettuce plants
Darqui, Flavia Soledad; Radonic, Laura Mabel; Trotz, Paulina M.; Lopez, Nilda Ester; Vazquez Rovere, Cecilia; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (Elsevier, 2018-10)Snakin-1 is a cysteine-rich antimicrobial peptide (AMP) isolated from potato tubers, with broad-spectrum activity. It belongs to the Snakin/GASA family, whose members have been studied because of their diverse roles ... -
Simplified methodology for large scale isolation of homozygous transgenic lines of lettuce
Darqui, Flavia Soledad; Radonic, Laura Mabel; Lopez, Nilda Ester; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (2018-01)Background: Lettuce is a globally important leafy vegetable and a model plant for biotechnology due to its adaptability to tissue culture and stable genetic transformation. Lettuce is also crucial for functional genomics ... -
The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
Darqui, Flavia Soledad; Hopp, Horacio Esteban; Lopez Bilbao, Marisa Gisela (Crimson Publishers, 2020-08)Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been ...