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Assessment on different vaccine formulation parameters in the protection against heterologous challenge with FMDV in cattle
Di Giacomo, Sebastián; Bucafusco, Danilo; Schammas, Juan Manuel; Pega, Juan Franco; Miraglia, Maria Cruz; Barrionuevo, Florencia Mabel; Capozzo, Alejandra; Perez Filgueira, Daniel Mariano (MDPI, 2022-08)Foot-and-mouth disease (FMD) remains one of the major threats to animal health worldwide. Its causative agent, the FMD virus (FMDV), affects cloven-hoofed animals, including farm animals and wildlife species, inflicting ... -
Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
Pega, Juan Franco; Bucafusco, Danilo; Di Giacomo, Sebastián; Schammas, Juan Manuel; Malacari, Darío Amilcar; Capozzo, Alejandra Victoria; Arzt, J.; Pérez-Beascoechea, C.; Maradei, E.; Rodríguez, L.L.; Borca, Manuel Victor; Perez Filgueira, Daniel Mariano (2013-03)Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase ... -
Efecto de altas presiones hidrostáticas sobre parámetros de calidad y microbiológicos en una bebida fermentada utilizando suero lácteo
Ambrosi, Vanina A.; Denoya, Gabriela Inés; Castells, María Laura; Sarquis, Sandra; Aranibar, Germán F.; Vaudagna, Sergio Ramon; Nanni, Mariana; Diaz, Gabriela Esther; Pega, Juan Franco (Facultad Regional Buenos Aires, Universidad Tecnológica Nacional, 2019-07)Evaluar el efecto de APH sobre parámetros de calidad y microbiológicos en una bebida fermentada, elaborada a partir de lactosuero, a fin de: - Proponer una alternativa de aprovechamiento de este subproducto mediante su ... -
Efecto de yogur fortificado con nanoliposomas de ácidos grasos Omega 3 (EPA+DHA) sobre índices nutricionales
Diaz, Gabriela Esther; Pega, Juan Franco; Perez, Carolina Daiana; Ambrosi, Vanina; Guidi, Silvina Mabel; Nanni, Mariana Sandra (Asociación Argentina de Tecnólogos Alimentarios (AATA), 2023-10)The main Omega3 polyunsaturated fatty acids (𝜔3 PUFA) are alpha-linolenic acid (ALA, 18:3 n-3), eicosapentaenoic acid (EPA, 202:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3). ALA is essential; It means that the body ... -
Effect of High-Pressure Processing on Quality and Microbiological Properties of a Fermented Beverage Manufactured from Sweet Whey Throughout Refrigerated Storage
Pega, Juan Franco; Denoya, Gabriela Inés; Castells, M.L.; Sarquis, S.; Aranibar, G.F.; Vaudagna, Sergio Ramon; Nanni, Mariana (2018-02)The production of fermented beverages is a promising way to valorize by-products of dairy manufacturing. However, the shelf-life of these products is often limited by the post-acidification process that occurs during ... -
Effect of Light Exposure on Functional Compounds of Monovarietal Extra Virgin Olive Oils and Oil Mixes During Early Storage as Evaluated by Fluorescence Spectra
Diaz, Gabriela Esther; Pega, Juan Franco; Primrose, D.; Sancho, Ana Maria; Nanni, Mariana (Springer, 2019-08-19)The oxidation of antioxidants in bottled extra virgin olive oil (EVOO), especially vitamin E and chlorophyll, can affect the quality of the product. However, the effect of light exposure on these functional compounds and ... -
Effect of the addition of phytosterols and tocopherols on Streptococcus thermophilus robustness during industrial manufacture and ripening of a functional cheese as evaluated by qPCR and RT-qPCR
Pega, Juan Franco; Rizzo, Sergio Anibal; Perez, Carolina Daiana; Rossetti, Luciana; Diaz, Gabriela; Ruzal, S.M.; Nanni, Mariana; Descalzo, Adriana Maria (2016-09)The quality of functional food products designed for the prevention of degenerative diseases can be affected by the incorporation of bioactive compounds. In many types of cheese, the performance of starter microorganisms ... -
Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
Kenney, Mary; Waters, Ryan A.; Rieder, Elizabeth; Pega, Juan Franco; Perez Filgueira, Daniel Mariano; Golde, William T. (Elsevier, 2017-11)Analysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been ... -
Evaluación de la incorporación de Omega-3 libre y nanoencapsulado en el desarrollo de un yogur
Diaz, Gabriela Esther; Pega, Juan Franco; Perez, Carolina Daiana; Ambrosi, Vanina; Guidi, Silvina Mabel; Nanni, Mariana (PUBLITEC S.A. Argentina, 2023-03)The health benefits derived from the consumption of omega-3 eicosapentaenoic (EPA) and docosahexaenoic (DHA) fatty acids have been strongly demonstrated in the scientific literature, and their consumption is recommended ... -
Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles
Bucafusco, Danilo; Di Giacomo, Sebastián; Pega, Juan Franco; Schammas, Juan Manuel; Cardoso, Nancy Patricia; Capozzo, Alejandra Victoria; Perez Filgueira, Daniel Mariano (2015-02)Interferon-γ (IFN-γ) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. Here, a recall IFN-γ assay based on a commercial ... -
Heterogeneity in the antibody response to foot‐and‐mouth disease primo‐vaccinated calves
Di Giacomo, Sebastián; Brito, Barbara Patricia; Perez, A.M.; Bucafusco, Danilo; Pega, Juan Franco; Rodríguez, L.; Borca, Manuel Victor; Perez Filgueira, Daniel Mariano (2015-06)Foot‐and‐mouth disease (FMD) vaccines are routinely used as effective control tools in large regions worldwide and to limit outbreaks during epidemics. Vaccine‐induced protection in cattle has been largely correlated with ... -
Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle
Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia Ana; Perez Filgueira, Daniel Mariano; Pega, Juan Franco; Gnazzo, Victoria; Romera, Sonia Alejandra; Schammas, Juan Manuel; Bucafusco, Danilo; Di Giacomo, Sebastián; García de la Torre, Beatriz; Andreu, David; Sobrino, Francisco; Blanco, Esther; Zamorano, Patricia Ines (2018-04)Synthetic peptides mimicking protective B- and T-cell epitopes are good candidates for safer, more effective FMD vaccines. Nevertheless, previous studies of immunization with linear peptides showed that they failed to ... -
Impact of extracellular nucleic acids from lactic acid bacteria on qPCR and RT-qPCR results in dairy matrices : implications for defining molecular markers of cell integrity
Pega, Juan Franco; Descalzo, Adriana Maria; Rossetti, Luciana; Nanni, Mariana; Perez, Carolina Daiana; Rizzo, Sergio Anibal; Diaz, Gabriela Perez (2017-07)In this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or ... -
Impacto de la incorporación de lípidos vegetales y antioxidantes sobre el comportamiento del starter durante la producción industrial de un queso funcional evaluado por técnicas independientes de cultivo
Pega, Juan Franco; Rizzo, Sergio Anibal; Rossetti, Luciana; Perez, Carolina Daiana; Diaz, Gabriela Esther; Ruzal, S.M.; Descalzo, Adriana Maria; Nanni, Mariana (2019)Los fitoesteroles son un conjunto de lípidos vegetales que han sido utilizados para el diseño de productos lácteos funcionales debido a su capacidad para reducir el colesterol sérico. No obstante, su efecto sobre el ... -
Influence of antibodies transferred by colostrum in the immune responses of calves to current foot-and-mouth disease vaccines
Bucafusco, Danilo; Di Giacomo, Sebastián; Pega, Juan Franco; Juncos, María Sol; Schammas, Juan Manuel; Perez Filgueira, Daniel Mariano; Capozzo, Alejandra Victoria (2014-11-12)Immunity to currently used oil-adjuvanted inactivated vaccines against foot-and-mouth disease virus (FMDV) has been studied in detail in adult animals; however, the influence of maternally derived antibodies transferred ... -
Systemic Foot-and-Mouth Disease Vaccination in Cattle Promotes Specific Antibody-Secreting Cells at the Respiratory Tract and Triggers Local Anamnestic Responses upon Aerosol Infection
Pega, Juan Franco; Di Giacomo, Sebastián; Bucafusco, Danilo; Schammas, Juan Manuel; Malacari, Darío Amilcar; Barrionuevo, Florencia Mariel; Capozzo, Alejandra Victoria; Rodríguez, L.L.; Borca, Manuel Victor; Perez Filgueira, Daniel Mariano (2015-09)Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting biungulate species. Commercial vaccines, formulated with inactivated FMD virus (FMDV), are regularly used worldwide to control the disease. Here, ... -
The incorporation of alpha-tocopherol and functional doses of phytosterol esters during cheesemaking does not affect DNA or mRNA dynamics of Streptococcus thermophilus and Lactococcus lactis throughout and after the end of ripening
Pega, Juan Franco; Descalzo, Adriana Maria; Rossetti, Luciana; Nanni, Mariana; Perez, Carolina Daiana; Rizzo, Sergio Anibal; Diaz, Gabriela; Ruzal, Sandra Monica (2017-10)Tocopherols and phytosterols are lipid-soluble molecules which have been widely used in the food industry. Nevertheless, the influence of these compounds on the performance of starter lactic acid bacteria (SLAB) in fermented ...