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Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S
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dc.contributor.author | Caminata Landriel, Soledad | |
dc.contributor.author | Castillo, Julieta D. L. M. | |
dc.contributor.author | Taboga, Oscar Alberto | |
dc.contributor.author | Ferrarotti, Susana Alicia | |
dc.contributor.author | Gottlieb, Alexandra Marina | |
dc.contributor.author | Costa, Hernán | |
dc.date.accessioned | 2020-01-14T13:30:43Z | |
dc.date.available | 2020-01-14T13:30:43Z | |
dc.date.issued | 2019-10 | |
dc.identifier.issn | 1678-2690 | |
dc.identifier.other | http://dx.doi.org/10.1590/0001-3765201920180568 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/6671 | |
dc.identifier.uri | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620&lng=en&nrm=iso&tlng=en | |
dc.description.abstract | Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Academia Brasileira de Ciências | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Anais da Academia Brasileira de Ciências 91 (3) : e20180568. (Octubre 2019) | por |
dc.subject | Paenibacillus | es_AR |
dc.subject | Glycosidases | eng |
dc.subject | Glicosidasas | es_AR |
dc.subject | Genetic Markers | eng |
dc.subject | Marcadores Genéticos | es_AR |
dc.subject | Enzyme Activity | eng |
dc.subject | Actividad Enzimática | es_AR |
dc.subject | Phylogeny | eng |
dc.subject | Filogenia | es_AR |
dc.title | Molecular identification of a cyclodextrin glycosyltransferase-producing microorganism and phylogenetic assessment of enzymatic activities | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Caminata Landriel, Soledad. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina | es_AR |
dc.description.fil | Fil: Castillo, Julieta D. L. M. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina | es_AR |
dc.description.fil | Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Laboratorio de Química Biológica; Argentina. | es_AR |
dc.description.fil | Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Costa, Hernán. Universidad Nacional de Luján. Departamento de Ciencias Básicas.Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.subtype | cientifico |
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