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This study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main terpene identified by gas chromatography electron impact mass spectrometry (GC-EIMS) was nerolidol, which was found in apical and basal tissues at a concentration
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dc.contributor.author | Escoriaza, Maria Georgina | |
dc.contributor.author | Garcia Lampasona, Sandra Claudia | |
dc.contributor.author | Gomez Talquenca, Gonzalo | |
dc.contributor.author | Piccoli, Patricia Noemí | |
dc.date.accessioned | 2019-08-23T13:06:25Z | |
dc.date.available | 2019-08-23T13:06:25Z | |
dc.date.issued | 2019-09 | |
dc.identifier.citation | Escoriaza, G., García Lampasona, S., Gomez Talquenca, S. et al. Plant Cell Tiss Organ Cult (2019) 138: 459. https://doi.org/10.1007/s11240-019-01641-3 | |
dc.identifier.issn | 0167-6857 | |
dc.identifier.issn | 1573-5044 (Online) | |
dc.identifier.other | https://doi.org/10.1007/s11240-019-01641-3 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/5679 | |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs11240-019-01641-3 | |
dc.description.abstract | This study investigated terpene biosynthesis in basal and apical tissues of in vitro-grown plants of Vitis vinifera cv. Chardonnay infected or not (control) with Phaeoacremonium parasiticum. This pathogen is one of the prevalent agents involved in the grapevine “hoja de malvón” disease. The main terpene identified by gas chromatography electron impact mass spectrometry (GC-EIMS) was nerolidol, which was found in apical and basal tissues at a concentration of ca. 0.12 μg mg FW−1. Consistent with this, an increment in terpene synthase (TPS) activity, assessed as tritiated farnesyl pyrophosphate ([1−3H]-FPP) transformed into hexane-soluble radioactive products in infected plants, was observed. TPS activity increased in correlation with the fungal concentration. Nerolidol inhibited in vitro mycelium growth and reduced fungal growth, in a concentration dependent manner. According to our results, the response of grapevine tissues to Pm. parasiticum is systemic, as it can be visualized by an augment of transcript abundance of VvPNLinNer1. It is also characterized by an increase of de novo synthesis of TPS responsible for the biosynthesis of phytoalexin nerolidol. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Plant cell, tissue and organ culture: PCTOC 138 (3) : 459–466. (September 2019) | eng |
dc.subject | Vitis Vinífera | es_AR |
dc.subject | Vitroplants | eng |
dc.subject | Vitroplantas | es_AR |
dc.subject | Fitoalexina | es_AR |
dc.subject | Phytoalexins | eng |
dc.subject | Respuesta Inmunológica | es_AR |
dc.subject | Immune Response | eng |
dc.subject | Infección | es_AR |
dc.subject | Infection | eng |
dc.subject | Enfermedades Fungosas | es_AR |
dc.subject | Fungal Diseases | eng |
dc.subject | Terpenoids | eng |
dc.subject | Terpenoidos | es_AR |
dc.subject.other | Phaeoacremonium Parasiticum | es_AR |
dc.subject.other | Nerolidol | es_AR |
dc.subject.other | Terpene Synthase Activity | eng |
dc.title | In vitro plants of Vitis vinifera respond to infection with the fungus Phaeoacremonium parasiticum by synthesizing the phytoalexin nerolidol | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | EEA Mendoza | es_AR |
dc.description.fil | Fil: Escoriaza, Maria Georgina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina | es_AR |
dc.description.fil | Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina | es_AR |
dc.description.fil | Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitopatología y Fitovirología; Argentina | es_AR |
dc.description.fil | Fil: Piccoli, Patricia Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina | es_AR |
dc.subtype | cientifico |
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