resumen
Abstract
The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to
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dc.contributor.author | Coniglio, Romina O. | |
dc.contributor.author | Fonseca, María I. | |
dc.contributor.author | Diaz, Gabriela V. | |
dc.contributor.author | Ontañon, Ornella Mailén | |
dc.contributor.author | Ghio, Silvina | |
dc.contributor.author | Campos, Eleonora | |
dc.contributor.author | Zapata, Pedro Darío | |
dc.date.accessioned | 2019-05-16T10:57:40Z | |
dc.date.available | 2019-05-16T10:57:40Z | |
dc.date.issued | 2019-04 | |
dc.identifier.issn | 2576-5299 | |
dc.identifier.other | https://doi.org/10.1080/25765299.2019.1598107 | |
dc.identifier.uri | https://www.tandfonline.com/doi/full/10.1080/25765299.2019.1598107 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/5122 | |
dc.description.abstract | The production of bioethanol from lignocellulosic biomass comprises the enzymatic hydrolysis of lignocellulosic structures by three major cellulases. Among them, cellobiohydrolases are considered to be key enzymes playing a significant role on cellulose degradation. The ability to produce lignocellulolytic enzymes by fungi such as Trametes villosa makes them appropriate degraders for large-scale applications. In this context, the aim of this study was to obtain and characterize a cellobiohydrolase-enriched extracellular extract of T. villosa LBM 033 (Misiones, Argentina), which is suitable for the enzymatic hydrolysis of lignocellulosic residues. The effect of carbon and nitrogen sources on cellobiohydrolase activity was evaluated using experimental designs and a culture medium was optimized to obtain a cellobiohydrolase-enriched extract suitable for the hydrolysis of lignocellulosic biomass. Moreover, by secretome analysis, nine enzymes involved in lignocellulosic biomass degradation were identified under the optimized conditions; among them is a cellobiohydrolase II from the glycosil-hydrolase 6 family. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Taylor & Francis | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Arab Journal of Basic and Applied Sciences 26 (1) : 182-192 (Abril 2019) | es_AR |
dc.subject | Enzymatic Hydrolysis | eng |
dc.subject | Hidrólisis Enzimática | es_AR |
dc.subject | Biomass | eng |
dc.subject | Biomasa | es_AR |
dc.subject | Trametes | es_AR |
dc.subject.other | Enzimas Lignocelulíticas | es_AR |
dc.subject.other | Lignocellulitic Enzymes | eng |
dc.subject.other | Bioetanol | es_AR |
dc.subject.other | Bioethanol | eng |
dc.title | Optimization of cellobiohydrolase production and secretome analysis of Trametes villosa LBM 033 suitable for lignocellulosic bioconversion | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Coniglio, Romina O. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Fonseca, María I. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Diaz, Gabriela V. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina | es_AR |
dc.description.fil | Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones. Laboratorio de Biotecnología Molecular; Argentina | es_AR |
dc.subtype | cientifico |
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