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Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
Abstract
To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision
[ver mas...]
To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis. These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well.
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Author
Kopertekh, Lilya;
Krebs, Erika;
Guzman, Fabiana;
Fuente
Plant biotechnology reports 12 (2) : 127–137. (April 2018)
Date
2018-04
Editorial
Springer
ISSN
1863-5466
1863-5474 (Online)
1863-5474 (Online)
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Restringido
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