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Capsicum comprises 35 species of chili peppers and five of them are cultivated worldwide as spices or vegetables. Diploid karyotypes based in x = 12 and x = 13 are common in Capsicum and the constitutive heterochromatin (cHet) is of particular interest in the genus since it is largely variable, particularly its highly GC-rich fraction. However, the repetitive DNA components of this heterochromatic regions are unknown. Given the co-localization of rDNA
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dc.contributor.author | Grabiele, Mauro | |
dc.contributor.author | Debat, Humberto Julio | |
dc.contributor.author | Scaldaferro, Marisel Analía | |
dc.contributor.author | Aguilera, Patricia Mabel | |
dc.contributor.author | Moscone, Eduardo Alberto | |
dc.contributor.author | Seijo, José Guillermo | |
dc.contributor.author | Ducasse, Daniel Adrian | |
dc.date.accessioned | 2018-10-29T17:47:35Z | |
dc.date.available | 2018-10-29T17:47:35Z | |
dc.date.issued | 2018-08 | |
dc.identifier.issn | 0304-4238 | |
dc.identifier.other | https://doi.org/10.1016/j.scienta.2018.04.060 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/3735 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0304423818303157?via%3Dihub#! | |
dc.description.abstract | Capsicum comprises 35 species of chili peppers and five of them are cultivated worldwide as spices or vegetables. Diploid karyotypes based in x = 12 and x = 13 are common in Capsicum and the constitutive heterochromatin (cHet) is of particular interest in the genus since it is largely variable, particularly its highly GC-rich fraction. However, the repetitive DNA components of this heterochromatic regions are unknown. Given the co-localization of rDNA loci with the CMA+DAPI– heterochromatic bands, we tested the hypothesis that the highly GC-rich cHet fraction is composed of the whole 18S-25S ribosomal DNA (rDNA) unit or some of its components. Here we report on a novel satellite for Capsicum and Solanaceae composed of the complete rDNA unit. We physically mapped six Capsicum derived specific 18S-25S rDNA probes that covered the entire span of the rDNA unit and analysed a DraI restriction product on eight chromosomally different taxa of Capsicum, representative of the major phylogenetic clades of chili peppers. The co-localization of every gene and spacer probes of the 18S-25S rDNA unit suggest their structural function as a major repetitive component of the highly GC-rich cHet in Capsicum species with x = 12. In addition, analyses of the clones derived from restriction assays in C. pubescens suggested that the differential functional status of 18S-25S rDNA loci (nucleolar organizer regions -NORs- or cHet) in this species is related to a divergence in a short sequence upstream the regulatory transcription initiation site (TIS) of the intergenic spacer (IGS). The results here provided evidence that an rDNA mega satellite played a significant role in the evolution of the karyotype features of x = 12 Capsicum species. The finding of a mega satellite family derived from the whole rDNA unit is a novelty for plant genomes | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.publisher | Elsevier | eng |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Scientia horticulturae 238 : 391-399. (19 August 2018) | eng |
dc.subject | Heterocromatina | es_AR |
dc.subject | Heterochromatin | eng |
dc.subject | Capsicum | es_AR |
dc.subject | Hibridación | es_AR |
dc.subject | Hybridization | eng |
dc.subject | Cytogenetics | eng |
dc.subject | Citogenetica | es_AR |
dc.subject.other | 18S-25S rDNA | eng |
dc.subject.other | Nucleolar Organizer Region | eng |
dc.subject.other | Fluorescence in Situ Hybridization | eng |
dc.subject.other | Cytogenetic Analysis | eng |
dc.title | Highly GC-rich heterochromatin in chili peppers (Capsicum-Solanaceae): A cytogenetic and molecular characterization | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.description.origen | Instituto de Patología Vegetal | es_AR |
dc.description.fil | Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Scaldaferro, Marisel Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina | es_AR |
dc.description.fil | Fil: Moscone, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste; Argentina | es_AR |
dc.description.fil | Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias. Laboratorio de Biología Molecular; Argentina | es_AR |
dc.subtype | cientifico |
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