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Resumen
Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). [ver mas...]
dc.contributor.authorPellegrini, María Celeste
dc.contributor.authorOkada, Elena
dc.contributor.authorGonzalez Pasayo, Ramon Alejandro
dc.contributor.authorPonce, Alejandra Graciela
dc.date.accessioned2022-11-10T14:21:40Z
dc.date.available2022-11-10T14:21:40Z
dc.date.issued2022-04
dc.identifier.issn1614-7499
dc.identifier.otherhttps://doi.org/10.1007/s11356-021-17523-1
dc.identifier.urihttp://hdl.handle.net/20.500.12123/13375
dc.identifier.urihttps://link.springer.com/article/10.1007/s11356-021-17523-1
dc.description.abstractEscherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). Phyllo-grouping assignment was performed on the total of E. coli isolates. Antibiograms and quantification of the minimal inhibitory concentration (MIC) were performed with antibiotics commonly used in humans. Biofilm formation capacity was studied by quantifying cells attached to culture tubes. Overall, 21 E. coli isolates were obtained. Three phylogenetic groups (A, B1, and C) and two Escherichia clade IV and IV–V were identified in the collection by polymerase chain reaction. Sixty-seven percent of the E. coli isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin. Amoxicillin MIC values ranged from 11.9 to >190.5 µg/mL and ampicillin MIC values ranged from 3 to >190.5 µg/mL. All the E. coli isolates, resistant and non-resistant, had biofilm forming capacity. The presence of phenotypic resistance on fresh produce and environmental matrices could present significant opportunities for contamination that result in health risks for consumers. To the authors’ best knowledge, this is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherSpringeres_AR
dc.relationinfo:eu-repograntAgreement/INTA/2019-PD-E2-I518-002/2019-PD-E2-I518-002/AR./Estudio del impacto ambiental, gestión y tratamiento de residuos y efluentes sobre sistemas agropecuarios y agroindustriales para su valorización agronómicaes_AR
dc.rightsinfo:eu-repo/semantics/restrictedAccesses_AR
dc.sourceEnvironmental Science and Pollution Research 29 : 23225-23236 (2022)es_AR
dc.subjectEscherichia Colies_AR
dc.subjectSueloes_AR
dc.subjectSoileng
dc.subjectAguas Subterráneases_AR
dc.subjectGroundwatereng
dc.subjectCama (animales)es_AR
dc.subjectLitter for Animalseng
dc.subjectAntibióticoses_AR
dc.subjectAntibioticseng
dc.subjectBiofilmes (microbiología)es_AR
dc.subjectBiofilms (microbiology)eng
dc.titlePrevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliationes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.description.origenEEA Balcarcees_AR
dc.description.filFil: Pellegrini, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.description.filFil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.es_AR
dc.description.filFil: González Pasayo, Ramón Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.es_AR
dc.description.filFil: Ponce, Alejandra Graciela. Universidad Nacional de Mar del Plata. Facultad de Ingeniería; Argentina. Comisión de Investigaciones Científicas; Argentina. Instituto de Ciencia y Tecnología de los alimentos; Argentina.es_AR
dc.description.filFil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.es_AR
dc.subtypecientifico


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