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Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines
Abstract
Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species.
Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a
[ver mas...]
Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species.
Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification.
Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm
and AMOVA analysis.
Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/ anteater isolate and the bovine group.
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![Thumbnail](/xmlui/bitstream/handle/20.500.12123/1117/ParasitesandVectors-logo.png?sequence=3&isAllowed=y)
Author
Guillemi, Eliana Carolina;
De La Fourniere, Sofia Ana María;
Orozco, Marcela;
Peña Martínez, Jorge;
Correa, Elena;
Fernández, Javier;
Lopez Arias, Ludmila Sol;
Paoletta, Martina;
Corona, Belkis;
Pinarello, Valérie;
Wilkowsky, Silvina Elizabeth;
Farber, Marisa Diana;
Fuente
Parasites & Vectors 169 : 305 (2016)
Date
2016
ISSN
1756-3305 (Online)
Formato
pdf
Tipo de documento
article
Palabras Claves
Derechos de acceso
Abierto
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