resumen
Abstract
This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase
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dc.contributor.author | Montoya, Marina Rosa | |
dc.contributor.author | Massa, Gabriela Alejandra | |
dc.contributor.author | Colabelli, Mabel Noemí | |
dc.contributor.author | Ridao, Azucena | |
dc.date.accessioned | 2021-09-13T13:16:34Z | |
dc.date.available | 2021-09-13T13:16:34Z | |
dc.date.issued | 2021-03-10 | |
dc.identifier.issn | 0167-7012 | |
dc.identifier.other | https://doi.org/10.1016/j.mimet.2021.106197 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/10234 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0167701221000658 | |
dc.description.abstract | This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | spa | es_AR |
dc.publisher | Elsevier | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/PNPV-1135024/AR./Defensa en plantas contra fitopatógenos. | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas. | |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Journal of Microbiological Methods 184 : 106197 (May 2021) | es_AR |
dc.subject | Soja | es_AR |
dc.subject | Soybeans | eng |
dc.subject | Tallos | es_AR |
dc.subject | Stems | eng |
dc.subject | Diaporthe | es_AR |
dc.subject | Agrobacterium Tumefaciens | es_AR |
dc.subject | Infecciones Latentes | es_AR |
dc.subject | Latent Infection | eng |
dc.subject.other | Diaporthe caulivora | |
dc.title | Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | EEA Balcarce | es_AR |
dc.description.fil | Fil: Montoya, Marina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. | es_AR |
dc.description.fil | Fil: Massa. Gabriela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. | es_AR |
dc.description.fil | Fil: Massa. Gabriela Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. | es_AR |
dc.description.fil | Fil: Colabelli, Mabel Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. | es_AR |
dc.description.fil | Fil: Ridao, Azucena del Carmen. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. | es_AR |
dc.subtype | cientifico |
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