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Genome-wide identification of microRNA targets in the neglected disease pathogens of the genus Echinococcus
Abstract
MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at posttranscriptional level and play essential roles in biological processes such as development. MiRNAs silence target mRNAs by binding to complementary sequences in the 3′untranslated regions (3′UTRs). The parasitic helminths of the genus Echinococcus are the causative agents of echinococcosis, a zoonotic neglected disease. In previous work, we performed a
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MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at posttranscriptional level and play essential roles in biological processes such as development. MiRNAs silence target mRNAs by binding to complementary sequences in the 3′untranslated regions (3′UTRs). The parasitic helminths of the genus Echinococcus are the causative agents of echinococcosis, a zoonotic neglected disease. In previous work, we performed a comprehensive identification and characterization of Echinococcus miRNAs. However, current knowledge about their targets is limited. Since target prediction algorithms rely on complementarity between 3′UTRs and miRNA sequences, a major limitation is the lack of accurate sequence information of 3′UTR for most species including parasitic helminths. We performed RNA-seq and developed a pipeline that integrates the transcriptomic data with available genomic data of this parasite in order to identify 3′UTRs of Echinococcus canadensis. The high confidence set of 3′UTRs obtained allowed the prediction of miRNA targets in Echinococcus through a bioinformatic approach. We performed for the first time a comparative analysis of miRNA targets in Echinococcus and Taenia. We found that many evolutionarily conserved target sites in Echinococcus and Taenia may be functional and under selective pressure. Signaling pathways such as MAPK and Wnt were among the
most represented pathways indicating miRNA roles in parasite growth and development. Genome-wide identification and characterization of miRNA target genes in Echinococcus provide valuable information to guide experimental studies in order to understand miRNA functions in the parasites biology. miRNAs involved in essential functions, especially those being absent in the host or showing sequence divergence with respect to host orthologs, might be considered as novel therapeutic targets for echinococcosis control.
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![Thumbnail](/bitstream/handle/20.500.12123/945/MolecularBiochemicalParasitology-cover.gif?sequence=2&isAllowed=y)
Author
Macchiaroli, Natalia;
Maldonado, Lucas;
Zarowiecki, Magdalena;
Cucher, Marcela Alejandra;
Gismondi, Maria Ines;
Kamenetzky, Laura;
Rosenzvit, Mara Cecilia;
Fuente
Molecular and biochemical parasitology 214 : 91-100. (2017)
Date
2017-06
ISSN
0166-6851
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Restringido
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