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A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected
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dc.contributor.author | Blanco, Federico Carlos | |
dc.contributor.author | Bianco, María Veronica | |
dc.contributor.author | Garbaccio, Sergio Gabriel | |
dc.contributor.author | Meikle, Virginia | |
dc.contributor.author | Gravisaco, María José | |
dc.contributor.author | Montenegro, Valeria Noely | |
dc.contributor.author | Alfonseca, Edgar | |
dc.contributor.author | Singh, Mahavir G. | |
dc.contributor.author | Barandiaran, Soledad | |
dc.contributor.author | Canal, Ana María | |
dc.contributor.author | Vagnoni, Lucas Emilio | |
dc.contributor.author | Buddle, Bryce Malcom | |
dc.contributor.author | Bigi, Fabiana | |
dc.contributor.author | Cataldi, Angel Adrian | |
dc.date.accessioned | 2020-11-02T15:59:25Z | |
dc.date.available | 2020-11-02T15:59:25Z | |
dc.date.issued | 2013-05 | |
dc.identifier.issn | 1472-9792 | |
dc.identifier.other | https://doi.org/10.1016/j.tube.2013.02.004 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/8172 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/abs/pii/S1472979213000127 | |
dc.description.abstract | A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Elsevier | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/AERG-232001/AR./Desarrollos biotecnológicos para el control de enfermedades infecciosas en animales de interés económico | es_AR |
dc.relation | info:eu-repograntAgreement/INTA/AEBIO-243512/AR./Búsqueda y caracterización de biomarcadores para el control de enfermedades de interés pecuario mediante el empleo de técnicas post genómicas. | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.source | Tuberculosis 93 (3) : 363-372 (May 2013) | es_AR |
dc.subject | Tuberculosis | es_AR |
dc.subject | Mycobacterium bovis Infections | eng |
dc.subject | Infección por Mycobacterium bovis | es_AR |
dc.subject | Live Vaccines | eng |
dc.subject | Vacuna Viva | es_AR |
dc.subject | Cattle | eng |
dc.subject | Ganado Bovino | es_AR |
dc.subject | Virulent Pathogens | eng |
dc.subject | Organismos Patógenos Virulentos | es_AR |
dc.title | Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina | es_AR |
dc.description.fil | Fil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Alfonseca, Edgar. Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; México | es_AR |
dc.description.fil | Fil: Singh, Mahavir G. Lionnex; Alemania | es_AR |
dc.description.fil | Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina | es_AR |
dc.description.fil | Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina | es_AR |
dc.description.fil | Fil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina | es_AR |
dc.description.fil | Fil: Buddle, Bryce Malcom. AgResearch. Hopkirk Research Institute; Nueva Zelanda | es_AR |
dc.description.fil | Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.subtype | cientifico |
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