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A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
Abstract
Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need
[ver mas...]
Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.
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![Thumbnail](/bitstream/handle/20.500.12123/7868/Journal%20of%20Veterinary%20Diagnostic%20Investigation_cover.png?sequence=4&isAllowed=y)
Fuente
Journal of Veterinary Diagnostic Investigation 32 (1) : 112-117 (Febrero 2020)
Date
2020-02
Editorial
Sage Publications
ISSN
1943-4936
1040-6387
1040-6387
Documentos Relacionados
Formato
pdf
Tipo de documento
artículo
Proyectos
(ver más)
INTA/PNSA/1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche.
INTA/PNSA/1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos.
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