Comparación entre técnicas de inoculación de Fusarium verticillioides en espigas de maíz

Abstract

Se compararon Técnicas de Inoculación (TI) de Fusarium verticillioides en espigas de maíz a través del comportamiento de 15 cultivares frente a la Incidencia (I%) y Severidad (S%) de la podredumbre. Se realizó un ensayo en la localidad de Camet (Gral. Pueyrredón, Buenos Aires), en 2006/07, en el que las plantas recibieron las siguientes TI: 1) aspersión de una Suspensión de Inóculo (SI) sobre los Estigmas (ASE), 2) inyección de una SI en el Canal de los estigmas (ICE), 3) inserción de un palillo colonizado con micelio (IE) y 4) control, sin inoculación (C). Para la I%, se detectó interacción híbrido x TI. El método IE produjo mayor I% en todos los híbridos. En cambio, no hubo interacción híbrido x TI para S%, ni diferencia entre híbridos. Hubo correlación significativa según la I% según la infección natural ocurrida en Camet durante 2006/07 y la obtenida por ASE, ICE y C. Aunque ASE simuló mejor a una infección natural, los resultados de I% mostraron que ICE permitiría una mejor evaluación de la podredumbre.

Inoculation techniques (TI) of Fusarium verticillioides on corn ears were compared through the incidence (I%) and severity (S%) showed by 15 cultivars. Experimentation was carried out in Camet (Gral. Pueyrredón, Buenos Aires), during 2006/07. The TI were: 1) aspersion of an aqueous inoculum (SI) onto the silk (ASE); 2) injection of SI into the silk channel (ICE), 3) insertion in the mid-ear of a toothpick colonized with mycelium (IE), 4) control without inoculation (C). No interaction hybrid x TI and variability among hybrids were found for S%. Inoculations caused more severe symptoms than C. For I% the interaction hybrid x TI was significant, suggesting that I% produced by TI depended on the hybrids. IE method produced the highest I% overall hybrids. A correlation was found in the hybrids’ order according to I%, when the data for the natural infection Inoculation techniques (TI) of Fusarium verticillioides on corn ears were compared through the incidence (I%) and severity (S%) showed by 15 cultivars. Experimentation was carried out in Camet (Gral. Pueyrredón, Buenos Aires), during 2006/07. The TI were: 1) aspersion of an aqueous inoculum (SI) onto the silk (ASE); 2) injection of SI into the silk channel (ICE), 3) insertion in the mid-ear of a toothpick colonized with mycelium (IE), 4) control without inoculation (C). No interaction hybrid x TI and variability among hybrids were found for S%. Inoculations caused more severe symptoms than C. For I% the interaction hybrid x TI was significant, suggesting that I% produced by TI depended on the hybrids. IE method produced the highest I% overall hybrids. A correlation was found in the hybrids’ order according to I%, when the data for the natural infection 2006/07 and ASE, ICE and C were compared. Although ASE showed the best simulation of a natural infection, ICE would permit the best evaluation of differences in F.verticillioides attack on ears. 006/07 and ASE, ICE and C were compared. Although ASE showed the best simulation of a natural infection, ICE would permit the best evaluation of differences in F.verticillioides attack on ears.