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Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum cultivar ‘Cayenne’ were treated with an acute dose of X-rays (300 Gy) and chromosome aberrations were analysed by different cytogenetic methods [Feulgen, silver staining for nucleolus organizer regions (silver positive nucleolus organizing
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dc.contributor.author | Scaldaferro, Marisel Analía | |
dc.contributor.author | Grabiele, Mauro | |
dc.contributor.author | Seijo, Jose Guillermo | |
dc.contributor.author | Debat, Humberto Julio | |
dc.contributor.author | Romero, María Victoria | |
dc.contributor.author | Ducasse, Daniel Adrian | |
dc.contributor.author | Prina, Alberto Raul | |
dc.contributor.author | Moscone, Eduardo Alberto | |
dc.date.accessioned | 2018-04-13T13:35:48Z | |
dc.date.available | 2018-04-13T13:35:48Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0955-3002 | |
dc.identifier.issn | 1362-3095 | |
dc.identifier.other | https://doi.org/10.3109/09553002.2013.825064 | |
dc.identifier.uri | https://www.tandfonline.com/doi/abs/10.3109/09553002.2013.825064 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/2242 | |
dc.description.abstract | Purpose: To locate transient chromosome aberrations on a selected pepper cultivar and determine the tracing efficiency of different cytogenetic methods. Materials and methods: Seeds from Capsicum baccatum var. pendulum cultivar ‘Cayenne’ were treated with an acute dose of X-rays (300 Gy) and chromosome aberrations were analysed by different cytogenetic methods [Feulgen, silver staining for nucleolus organizer regions (silver positive nucleolus organizing regions or AgNOR), fluorescent banding, fluorescence in situ hybridization (FISH) and meiotic analysis]. Results: A rearranged chromosome carrying two nucleolus organizing regions (NOR) induced by ionizing radiation was detected in the cultivar, with the occurrence of a small reciprocal exchange between a chromosome of pair no. 1 and another chromosome of pair no. 3, both carrying active NOR in short arms and associated chromomycin A positive/diamidino-phenylindole negative (CMA+/DAPI−) heterochromatin. Meiotic analysis showed a quadrivalent configuration, confirming a reciprocal translocation between two chromosomes. Conclusions: The use of X-rays in Capsicum allowed us to develop and identify a pepper line with structural rearrangements between two NOR-carrying chromosomes. We postulate that all the cytological techniques employed in this research were efficient in the search for chromosome aberrations. Particularly, Feulgen and AgNOR were the most suitable in those cases of transient rearrangements, whereas fluorescent banding and FISH were appropriate for intransitive ones. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.rights | info:eu-repo/semantics/openAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | International Journal of Radiation Biology 90 (1) : 104-112 (2014) | es_AR |
dc.subject | Capsicum baccatum | es_AR |
dc.subject | Guindilla | es_AR |
dc.subject | Chillies | eng |
dc.subject | Cromosomas | es_AR |
dc.subject | Chromosomes | eng |
dc.subject | Citogenética | es_AR |
dc.subject | Cytogenetics | eng |
dc.subject | Radiación Ionizante | es_AR |
dc.subject | Ionizing Radiation | eng |
dc.subject.other | Capsicum pendulum | es_AR |
dc.title | Efficiency of cytogenetic methods in detecting a chromosome rearrangement induced by ionizing radiation in a cultivated chili pepper line (Capsicum baccatum var. pendulum – Solanaceae) | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/acceptedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Instituto de Patología Vegetal | es_AR |
dc.description.origen | Instituto de Genética | |
dc.description.fil | Fil: Scaldaferro, Marisel Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste; Argentina | es_AR |
dc.description.fil | Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Romero, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | es_AR |
dc.description.fil | Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina | es_AR |
dc.description.fil | Fil: Moscone, Eduardo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina | es_AR |
dc.subtype | cientifico |
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