resumen
Abstract
Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites
and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in
signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic
approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the
[ver mas...]
dc.contributor.author | Bazzini, Ariel Alejandro | |
dc.contributor.author | Manacorda, Carlos Augusto | |
dc.contributor.author | Tohge, Takayuki | |
dc.contributor.author | Conti, Gabriela | |
dc.contributor.author | Rodriguez, Maria Cecilia | |
dc.contributor.author | Nunes Nesi, Adriano | |
dc.contributor.author | Villanueva, Sofía | |
dc.contributor.author | Fernie, Alisdair R. | |
dc.contributor.author | Carrari, Fernando | |
dc.contributor.author | Asurmendi, Sebastian | |
dc.date.accessioned | 2018-04-04T18:21:36Z | |
dc.date.available | 2018-04-04T18:21:36Z | |
dc.date.issued | 2011-12-12 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/2168 | |
dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236191/ | |
dc.description.abstract | Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration. | eng |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.rights | info:eu-repo/semantics/openAccess | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | PLoS ONE 6 (12) :e28466. (December 2011) | eng |
dc.subject | RNA | es_AR |
dc.subject | Metabolismo | es_AR |
dc.subject | Virus de las Plantas | es_AR |
dc.subject | Nicotiana Tabacum | es_AR |
dc.subject | Tabaco | es_AR |
dc.subject | Cromatografía Gas-líquido | es_AR |
dc.subject | Espectrometría de Masas | es_AR |
dc.subject | Mass Spectrometry | eng |
dc.subject | Gas Liquid Chromatography | eng |
dc.subject | Tobacco | eng |
dc.subject | Plant Viruses | |
dc.subject.other | Virus del Mosaico del Tabaco | es_AR |
dc.subject.other | miRNA | es_AR |
dc.title | Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes | eng |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/publishedVersion | eng |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.fil | Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Tohge, Takayuki. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania | es_AR |
dc.description.fil | Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Nunes Nesi, Adriano. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania | es_AR |
dc.description.fil | Fil: Villanueva, Sofía. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.description.fil | Fil: Fernie, Alisdair R. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania | es_AR |
dc.description.fil | Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | es_AR |
dc.subtype | cientifico |
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