Listar Instituto de Biotecnología por fecha de publicación
Mostrando ítems 21-40 de 409
-
Coupling virus-induced gene silencing to exogenous green fluorescence protein expression provides a highly efficient system for functional genomics in arabidopsis and across all stages of tomato fruit development
(2011-07)Since the advent of the postgenomic era, efforts have focused on the development of rapid strategies for annotating plant genes of unknown function. Given its simplicity and rapidity, virus-induced gene silencing (VIGS) ... -
Genetic dissection of vitamin E biosynthesis in tomato
(Society for Experimental Biology, 2011-07)Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate ... -
Respuesta inmune en terneros experimentalmente infectados con diferentes dosis de Mycobacterium bovis
(Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2011-08)La tuberculosis bovina es una enfermedad zoonótica producida por Mycobacterium bovis que afecta a animales de producción causando pérdidas económicas y representa, además, un problema para la salud pública. El conocimiento ... -
An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
(Sage Publishing, 2011-08)Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for ... -
Nuevas estrategias para la transformación y expresión de genes de interés en girasol
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2011-09)El girasol es una especie de la familia Asteraceae (Compositae) de gran importancia económica, considerada hasta hace una década recalcitrante al cultivo in vitro y la transformación genética. En un trabajo previo (Radonic, ... -
Development of a novel set of Gateway-compatible vectors for live imaging in insect cells
(The Royal Entomological Society, 2011-10)Insect genomics is a growing area of research. To exploit fully the genomic data that are being generated, high-throughput systems for the functional characterization of insect proteins and their interactomes are required. ... -
Reference gene selection for gene expression studies using RT-qPCR in virus-infected planthoppers
(BioMed Central, 2011-12)Background: Planthoppers not only severely affect crops by causing mechanical damage when feeding but are also vectors of several plant virus species. The analysis of gene expression in persistently infected planthoppers ... -
Metabolic and miRNA profiling of TMV infected plants reveals biphasic temporal changes
(2011-12-12)Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways ... -
Snakin/GASA proteins : involvement in hormone crosstalk and redox homeostasis
(Taylor & Francis, 2012)Snakin/GASA proteins are widely distributed among plant species. They are expressed in different plant organs with high tissue and temporal specificity, and their subcellular localization varies among the different members. ... -
Transgenic expression of tobacco mosaic virus capsid and movement proteins modulate plant basal defense and biotic stress responses in Nicotiana tabacum
(2012)Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic ... -
Potato Snakin-1 Gene Silencing Affects Cell Division, Primary Metabolism, and Cell Wall Composition
(American Society of Plant Biologists, 2012-01)Snakin-1 (SN1) is an antimicrobial cysteine-rich peptide isolated from potato (Solanum tuberosum) that was classified as a member of the Snakin/Gibberellic Acid Stimulated in Arabidopsis protein family. In this work, a ... -
Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
(S. Karger AG, 2012-02)Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On ... -
Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
(Mary Ann Liebert Inc., 2012-02)Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we ... -
Uso de Brucella abortus como vector para la expresión de antígenos heterólogos
(Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2012-03)La brucelosis es una zoonosis bacteriana causada por especies del género Brucella. B. abortus S19 es la cepa vacunal en uso en nuestro país. Debido a su capacidad para despertar una respuesta inmune celular y humoral, B. ... -
Genetic diversity of maize landraces from lowland and highland agro‐ecosystems of Southern South America: implications for the conservation of native resources
(Wiley, 2012-05)The North of Argentina is one of the southernmost areas of maize landrace cultivation. Two distinct centres of diversity have been distinguished within this region: Northwestern Argentina (NWA), and Northeastern Argentina ... -
Association mapping in sunflower for sclerotinia head rot resistance
(BMC, 2012-06)Background: Sclerotinia Head Rot (SHR) is one of the most damaging diseases of sunflower in Europe, Argentina, and USA, causing average yield reductions of 10 to 20 %, but leading to total production loss under favorable ... -
Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment
(2012-08)Phenotypic plasticity is often postulated as a principal characteristic of tuber-bearing wild Solanum species. The hypotheses to explore this observation have been developed based on the presence of genetic variation. In ... -
AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny
(Plos One, 2012-09)The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of ... -
In vivo subcellular localization of Mal de Río Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions
(Elsevier, 2012-09)The in vivo subcellular localization of Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution ... -
Development, characterization and experimental validation of a cultivated sunflower (Helianthus annuus L.) gene expression oligonucleotide microarray
(PLOS One, 2012-10)Oligonucleotide-based microarrays with accurate gene coverage represent a key strategy for transcriptional studies in orphan species such as sunflower, H. annuus L., which lacks full genome sequences. The goal of this study ...