resumen
Abstract
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC
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dc.contributor.author | Su, Yun‑Lin | |
dc.contributor.author | Larzabal, Mariano | |
dc.contributor.author | Song, Huan | |
dc.contributor.author | Cheng, Tianfang | |
dc.contributor.author | Wang, Yufang | |
dc.contributor.author | Smith, Libia Yael | |
dc.contributor.author | Cataldi, Angel Adrian | |
dc.contributor.author | Ow, David W. | |
dc.date.accessioned | 2023-10-24T15:00:03Z | |
dc.date.available | 2023-10-24T15:00:03Z | |
dc.date.issued | 2023-10 | |
dc.identifier.issn | 1432-2242 | |
dc.identifier.other | https://doi.org/10.1007/s00122-023-04460-5 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/15662 | |
dc.identifier.uri | https://link.springer.com/article/10.1007/s00122-023-04460-5 | |
dc.description.abstract | Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen where ruminant farm animals, mainly bovine, serve as reservoirs. Bovine vaccination has been used to prevent disease outbreaks, and the current method relies on vaccines subcutaneously injected three times per year. Since EHEC O157:H7 colonizes mucosal surfaces, an oral vaccine that produces an IgA response could be more convenient. Here, we report on oral vaccination against EHEC O157:H7 in mice orally gavaged with transgenic lettuce that produces EHEC O157:H7 antigens EspB and γ-intimin C280. Younger leaves accumulated a higher concentration of antigens; and in unexpanded leaves of 30-day-old T2 plants, EspB and γ-intimin C280 were up to 32 and 51 μg/g fresh weight, respectively. Mice orally gavaged with lettuce powders containing < 3 µg antigens for 6 days showed a mucosal immune response with reduced colonization of EHEC O157:H7. This suggests that the transgenic lettuce has potential to be used for bovine vaccination. To promote the biosafety of crop plants producing medically relevant proteins, recombination sites were built into our transgenic lines that would permit optional marker removal by Cre-lox recombination, as well as transgene deletion in pollen by CinH-RS2 recombination. The ability to upgrade the transgenic lettuce by stacking additional antigen genes or replacing older genes with newer versions would also be possible through the combined use of Bxb-att and Cre-lox recombination systems. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | Springer | es_AR |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | Theoretical and Applied Genetics 136 (10) : 214 (Octubre 2023) | es_AR |
dc.subject | Escherichia coli | es_AR |
dc.subject | Antigens | eng |
dc.subject | Antígenos | es_AR |
dc.subject | Lettuces | eng |
dc.subject | Lechugas | es_AR |
dc.subject | Vaccines | eng |
dc.subject | Vacuna | es_AR |
dc.subject | Mice | eng |
dc.subject | Ratón | es_AR |
dc.subject | Transgenes | eng |
dc.title | Enterohemorrhagic Escherichia coli O157:H7 antigens produced in transgenic lettuce effective as an oral vaccine in mice | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | Instituto de Biotecnología | es_AR |
dc.description.fil | Fil: Su, Yun‑Lin. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China | es_AR |
dc.description.fil | Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Song, Huan. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China | es_AR |
dc.description.fil | Fil: Cheng, Tianfang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China | es_AR |
dc.description.fil | Fil: Wang, Yufang. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China | es_AR |
dc.description.fil | Fil: Smith, Libia Yael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Smith, Libia Yael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina | es_AR |
dc.description.fil | Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es_AR |
dc.description.fil | Fil: Ow, David W. Chinese Academy of Sciences. South China Botanical Garden. Guangdong Provincial Key Laboratory of Applied Botany. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement. Plant Gene Engineering Center; República Popular China | es_AR |
dc.subtype | cientifico |
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