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The cytosolic invertase NI6 affects vegetative growth, flowering, fruit set, and yield in tomato
Abstract
Sucrose metabolism is important for most plants, both as the main source of carbon and via signaling mechanisms that have been proposed for this molecule. A cleaving enzyme, invertase (INV) channels sucrose into sink metabolism. Although acid soluble and insoluble invertases have been largely investigated, studies on the role of neutral invertases (A/N-INV) have lagged behind. Here, we identified a tomato A/N-INV encoding gene (NI6) co-localizing with a
[ver mas...]
Sucrose metabolism is important for most plants, both as the main source of carbon and via signaling mechanisms that have been proposed for this molecule. A cleaving enzyme, invertase (INV) channels sucrose into sink metabolism. Although acid soluble and insoluble invertases have been largely investigated, studies on the role of neutral invertases (A/N-INV) have lagged behind. Here, we identified a tomato A/N-INV encoding gene (NI6) co-localizing with a previously reported quantitative trait locus (QTL) largely affecting primary carbon metabolism in tomato. Of the eight A/N-INV genes identified in the tomato genome, NI6 mRNA is present in all organs, but its expression was higher in sink tissues (mainly roots and fruits). A NI6-GFP fusion protein localized to the cytosol of mesophyll cells. Tomato NI6-silenced plants showed impaired growth phenotype, delayed flowering and a dramatic reduction in fruit set. Global gene expression and metabolite profile analyses of these plants revealed that NI6 is not only essential for sugar metabolism, but also plays a signaling role in stress adaptation. We also identified major hubs, whose expression patterns were greatly affected by NI6 silencing; these hubs were within the signaling cascade that coordinates carbohydrate metabolism with growth and development in tomato.
[Cerrar]
Author
Coluccio Leskow, Carla;
Conte, Mariana;
del Pozo, Talía;
Bermudez Salazar, Luisa;
Lira, Bruno Silvestre;
Gramegna, Giovanna;
Baroli, Irene;
Burgos, Estanislao;
Zavallo, Diego;
Kamenetzky, Laura;
Asis, Ramón;
Gonzalez, Maurıcio;
Fernie, Alisdair R.;
Rossi, Magdalena;
Osorio, Sonia;
Carrari, Fernando;
Fuente
Journal of Experimental Botany 72 (7) : 2525-2543 (Marzo 2021)
Date
2021-03
Editorial
Oxford University Press
ISSN
1460-2431
Formato
pdf
Tipo de documento
artículo
Palabras Claves
Derechos de acceso
Restringido
Excepto donde se diga explicitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)