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Resumen
Fruit softening has been largely associated with cell wall degradation by a number of loosening proteins. Firmness is a major quality attribute of fresh cherries and also an important factor affecting the susceptibility to postharvest rots. By analyzing the solubilization, depolymerization and composition of pectins and hemicelluloses in cultivars with contrasting firmness we found that the pattern and
extent of their wall disassembly was quite similar.
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dc.contributor.author | Vicente, Ariel Roberto | |
dc.contributor.author | Rodoni, Luis Maria | |
dc.contributor.author | Salato, Gloria Silvia | |
dc.contributor.author | Basanta, Maria Florencia | |
dc.contributor.author | Ponce, Nora Marta Andrea | |
dc.contributor.author | Stortz, Carlos Arturo | |
dc.contributor.author | Raffo Benegas, Maria Dolores | |
dc.contributor.author | Powell, Ann L.T. | |
dc.contributor.author | Bennett, A.B. | |
dc.contributor.author | Labavitch, John Marcus | |
dc.date.accessioned | 2024-04-17T11:53:17Z | |
dc.date.available | 2024-04-17T11:53:17Z | |
dc.date.issued | 2015-03-25 | |
dc.identifier.issn | 0567-7572 | |
dc.identifier.other | https://doi.org/10.17660/ActaHortic.2015.1079.5 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/17434 | |
dc.description | Trabajo presentado al V International Conference Postharvest Unlimited, Chipre, 2015 | |
dc.description.abstract | Fruit softening has been largely associated with cell wall degradation by a number of loosening proteins. Firmness is a major quality attribute of fresh cherries and also an important factor affecting the susceptibility to postharvest rots. By analyzing the solubilization, depolymerization and composition of pectins and hemicelluloses in cultivars with contrasting firmness we found that the pattern and extent of their wall disassembly was quite similar. No marked pectin or hemicellulose depolymerization was observed and a similar reduction in tightly bound pectins and hemicellulose was detected in both cultivars during ripening. However, firm cherries presented pectic polymers with lower proportion of neutral sugars compared to uronic acids, suggesting that the variation in total wall polysaccharide and the branching of pectins assembled early in development or the proportion of homogalacturonan (HG) to type rhamnogalacturonan-I (RG-I) may contribute to the differences in firmness between cultivars. Dismantling of the cell wall by the action of relatively “well known” loosening agents is involved in the progressive softening occurring during ripening. Two of these proteins include polygalacturonases (PG) which are known to hydrolyze homogalacturonans and expansins (Exp) believed to participate in the relaxation of the cell wall by reducing hydrogen bonding between cellulose microfibrils and xyloglucan. We investigated the in vivo roles of these wall-disassembling proteins, by overexpressing PG and Exp1 both alone and in combination in a non-ripening rin tomato background. The simultaneous overexpression of PG and Exp1 in rin fruit restored softening in these non-ripening fruit. Unexpectedly, PG overexpression resulted in higher hemicellulose depolymerization while increased levels of Exp1 accelerated pectin turnover. This shows that besides their “well known” in vitro functions these proteins act in muro by facilitating the degradation of non directly targeted wall components, likely by increasing the accessibility of pre-existing wall-degrading proteins to their polysaccharide substrates. | eng |
dc.format | application/pdf | es_AR |
dc.language.iso | eng | es_AR |
dc.publisher | International Society for Horticultural Science | |
dc.rights | info:eu-repo/semantics/restrictedAccess | es_AR |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | es_AR |
dc.source | Acta Horticulturae 1079 : 61-69 (2015) | es_AR |
dc.subject | Fruits | eng |
dc.subject | Frutas | es_AR |
dc.subject | Cell Walls | eng |
dc.subject | Pared Celular | es_AR |
dc.subject | Polygalacturonase | eng |
dc.subject | Poligalacturonasa | es_AR |
dc.subject.other | Softening | eng |
dc.subject.other | Reblandecimiento | es_AR |
dc.subject.other | Expansin | eng |
dc.subject.other | Expansina | es_AR |
dc.title | Role of "well known" proteins on cell wall degradation and softening | es_AR |
dc.type | info:ar-repo/semantics/artículo | es_AR |
dc.type | info:eu-repo/semantics/article | es_AR |
dc.type | info:eu-repo/semantics/publishedVersion | es_AR |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | es_AR |
dc.description.origen | EEA Alto Valle | es_AR |
dc.description.fil | Fil: Vicente, Ariel Roberto. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina | es_AR |
dc.description.fil | Fil: Rodoni, Luis Maria. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina | es_AR |
dc.description.fil | Fil: Salato, Gloria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina | es_AR |
dc.description.fil | Fil: Basanta, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina | es_AR |
dc.description.fil | Fil: Ponce, Nora Marta Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina | es_AR |
dc.description.fil | Fil: Stortz, Carlos Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina | es_AR |
dc.description.fil | Fil: Raffo Benegas, María Dolores. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina | es_AR |
dc.description.fil | Fil: Powell, Ann L.T. University of California. Department of Plant Sciences, Estados Unidos | es_AR |
dc.description.fil | Fil: Bennett, A.B. University of California. Department of Plant Sciences, Estados Unidos | es_AR |
dc.description.fil | Fil: Labavitch, John Marcus. University of California. Department of Plant Sciences, Estados Unidos | es_AR |
dc.subtype | cientifico |
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