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resumen

Resumen
The role of water buffaloes in foot-and-mouth disease (FMD) epidemiology as one of the major hosts of the virus that can develop persistent asymptomatic infection highlights the importance of sustaining surveillance on the antibody response elicited by vaccination in these animals. There is gap in the knowledge on how serological assays that measure antibodies against capsid proteins perform with buffalo samples and which would be the most reliable test [ver mas...]
dc.contributor.authorSala, Juan Manuel
dc.contributor.authorMansilla, Florencia Celeste
dc.contributor.authorMiraglia, Maria Cruz
dc.contributor.authorCaspe, Sergio Gaston
dc.contributor.authorPerez Filgueira, Daniel Mariano
dc.contributor.authorCapozzo, Alejandra
dc.date.accessioned2024-04-15T11:39:13Z
dc.date.available2024-04-15T11:39:13Z
dc.date.issued2023-11
dc.identifier.issn2297-1769
dc.identifier.otherhttps://doi.org/10.3389/fvets.2023.1162477
dc.identifier.urihttp://hdl.handle.net/20.500.12123/17408
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fvets.2023.1162477/full
dc.description.abstractThe role of water buffaloes in foot-and-mouth disease (FMD) epidemiology as one of the major hosts of the virus that can develop persistent asymptomatic infection highlights the importance of sustaining surveillance on the antibody response elicited by vaccination in these animals. There is gap in the knowledge on how serological assays that measure antibodies against capsid proteins perform with buffalo samples and which would be the most reliable test to substitute the virus neutralization test (VNT) a cumbersome and low-throughput tool for field surveillance. Alternatively, the liquid-phase blocking sandwich ELISA (LPBE) is commonly used. Previous data from our laboratory demonstrated that the vaccine-induced antibodies assessed by the LPBE yielded low specificity with buffaloes’ samples. In contrast, a single-dilution avidity ELISA (AE) aimed to detect high-avidity antibodies against exposed epitopes, combined with an indirect ELISA (IE) to assess IgG levels, produced more reliable results. Here we analyzed for the first time the kinetics of the antibodies induced by vaccination in two different buffalo herds (n = 91) over 120 days using AE, IE, LPBE, and the VNT. Kinetics were similar in the different assays, with an increase of antibodies between 0- and 14-days post-vaccination (dpv) which were maintained thereafter. VNT and AE results were concordant (Kappa value = 0.76), and both assays revealed a decay in the antibody response in calves with maternal antibodies at 90 and 120 dpv, which was not evidenced by the LPBE. These results show that kinetics of antibody responses to FMD vaccination are similar in buffalo and cattle, and support the use of indirect ELISA assays, in particular Avidity ELISA, as alternatives to the VNT for vaccine-immunity monitoring irrespectively of the animal’s passive or active immune status.eng
dc.formatapplication/pdfes_AR
dc.language.isoenges_AR
dc.publisherFrontiers Mediaes_AR
dc.relationinfo:eu-repograntAgreement/INTA/PNSA/1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche.es_AR
dc.rightsinfo:eu-repo/semantics/openAccesses_AR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/es_AR
dc.sourceFrontiers in Veterinary Science 10 : 1162477. (November 2023)es_AR
dc.subjectBúfalo de Aguaes_AR
dc.subjectWater Buffaloeseng
dc.subjectEnfermedades de los Animaleses_AR
dc.subjectAnimal Diseaseseng
dc.subjectFiebre Aftosaes_AR
dc.subjectFoot and Mouth Diseaseeng
dc.subjectVacunaes_AR
dc.subjectVaccineseng
dc.subjectELISAeng
dc.subjectAnticuerposes_AR
dc.subjectAntibodieseng
dc.subjectSerologíaes_AR
dc.subjectSerologyeng
dc.titleKinetics of foot-and-mouth disease vaccine-induced antibody responses in buffaloes (Bubalus bubalis): avidity ELISA as an alternative to the virus neutralization testes_AR
dc.typeinfo:ar-repo/semantics/artículoes_AR
dc.typeinfo:eu-repo/semantics/articlees_AR
dc.typeinfo:eu-repo/semantics/publishedVersiones_AR
dc.rights.licenseCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)es_AR
dc.description.origenEEA Mercedeses_AR
dc.description.filFil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Miraglia, María Cruz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Miraglia, María Cruz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentinaes_AR
dc.description.filFil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Perez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.description.filFil: Capozzo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Virología e Innovaciones Tecnológicas; Argentinaes_AR
dc.subtypecientifico


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