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Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to
comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by
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dc.contributor.author | De Haro, Luis Alejandro | |
dc.contributor.author | Dumon, Analia Delina | |
dc.contributor.author | Mattio, Maria Fernanda | |
dc.contributor.author | Arguello Caro, Evangelina Beatriz | |
dc.contributor.author | Llauger, Gabriela | |
dc.contributor.author | Zavallo, Diego | |
dc.contributor.author | Blanc, Hervé | |
dc.contributor.author | Mongelli, Vanesa Claudia | |
dc.contributor.author | Truol, Graciela Ana Maria | |
dc.contributor.author | Saleh, María-Carla | |
dc.contributor.author | Asurmendi, Sebastian | |
dc.contributor.author | Del Vas, Mariana | |
dc.date.accessioned | 2017-09-01T18:02:06Z | |
dc.date.available | 2017-09-01T18:02:06Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | de Haro LA, Dumón AD, Mattio MF, Argüello Caro EB, Llauger G, Zavallo D, Blanc H, Mongelli VC, Truol G, Saleh M-C, Asurmendi S and del Vas M (2017) Mal de Río Cuarto Virus Infection Triggers the Production of Distinctive Viral-Derived siRNA Profiles in Wheat and Its Planthopper Vector. Front. Plant Sci. 8:766 | |
dc.identifier.other | https://doi.org/10.3389/fpls.2017.00766 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/1108 | |
dc.identifier.uri | http://journal.frontiersin.org/article/10.3389/fpls.2017.00766/full | |
dc.description.abstract | Plant reoviruses are able to multiply in gramineae plants and delphacid vectors encountering different defense strategies with unique features. This study aims to comparatively assess alterations of small RNA (sRNA) populations in both hosts upon virus infection. For this purpose, we characterized the sRNA profiles of wheat and planthopper vectors infected by Mal de Río Cuarto virus (MRCV, Fijivirus, Reoviridae) and quantified virus genome segments by quantitative reverse transcription PCR We provide evidence that plant and insect silencing machineries differentially recognize the viral genome, thus giving rise to distinct profiles of virus-derived small interfering RNAs (vsiRNAs). In plants, most of the virus genome segments were targeted preferentially within their upstream sequences and vsiRNAs mapped with higher density to the smaller genome segments than to the medium or larger ones. This tendency, however, was not observed in insects. In both hosts, vsiRNAs were equally derived from sense and antisense RNA strands and the differences in vsiRNAs accumulation did not correlate with mRNAs accumulation. We also established that the piwi-interacting RNA (piRNA) pathway was active in the delphacid vector but, contrary to what is observed in virus infected mosquitoes, virus-specific piRNAs were not detected. This work contributes to the understanding of the silencing response in insect and plant hosts. | eng |
dc.format | application/pdf | |
dc.language.iso | eng | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | |
dc.source | Frontiers in plant science 8 :766. (May 2017) | |
dc.subject | ARN | |
dc.subject | Virus de las Plantas | |
dc.subject | Plant Viruses | eng |
dc.subject | Trigo | |
dc.subject | Wheat | eng |
dc.subject | RNA | eng |
dc.subject | Fulgoroidea | |
dc.subject.other | Virus del Mal de Río Cuarto | |
dc.subject.other | Fijivirus | |
dc.title | Mal de Río Cuarto Virus infection triggers the production of distinctive viral-derived siRNA profiles in wheat and Its planthopper vector | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.rights.license | Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | |
dc.description.origen | Inst. de Biotecnología | |
dc.gic | 154168 | |
dc.description.fil | Fil: De Haro, Luis Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Dumon, Analia Delina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | |
dc.description.fil | Fil: Mattio, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | |
dc.description.fil | Fil: Arguello Caro, Evangelina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | |
dc.description.fil | Fil: Llauger, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | |
dc.description.fil | Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | |
dc.description.fil | Fil: Blanc, Hervé. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia | |
dc.description.fil | Fil: Truol, Graciela Ana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina | |
dc.description.fil | Fil: Mongelli, Vanesa Claudia. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia | |
dc.description.fil | Fil: Saleh, María-Carla. Centre National de la Recherche Scientifique. Institut Pasteur, Viruses and RNA Interference Unit; Francia | |
dc.description.fil | Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.subtype | cientifico |
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