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To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed,
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dc.contributor.author | Zanetti, Flavia Adriana | |
dc.contributor.author | Cardona, Romina | |
dc.contributor.author | Federico, Carlos Rodolfo | |
dc.contributor.author | Chimeno Zoth, Silvina Andrea | |
dc.contributor.author | Calamante, Gabriela | |
dc.date.accessioned | 2017-08-28T13:20:59Z | |
dc.date.available | 2017-08-28T13:20:59Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1995-820X (Online) | |
dc.identifier.issn | 1674-0769 (Print) | |
dc.identifier.other | https://doi.org/10.1007/s12250-015-3680-6 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12123/1051 | |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs12250-015-3680-6 | |
dc.description.abstract | To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′). | |
dc.format | application/pdf | eng |
dc.language.iso | eng | |
dc.rights | info:eu-repo/semantics/restrictedAccess | eng |
dc.source | Virologica sinica 31(3) : 266–269. (June 2016) | eng |
dc.subject | Enfermedades de los Animales | |
dc.subject | Pollo | |
dc.subject | Chickens | eng |
dc.subject | Animal Diseases | eng |
dc.subject | Animal Viruses | eng |
dc.subject | Virus de los Animales | |
dc.subject.other | Canarypox | |
dc.title | Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens | eng |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/article | eng |
dc.type | info:eu-repo/semantics/acceptedVersion | eng |
dc.description.origen | Inst. de Biotecnología | |
dc.gic | 152257 | |
dc.description.fil | Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina | |
dc.description.fil | Fil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina | |
dc.description.fil | Fil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: Argentina | |
dc.description.fil | Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | |
dc.description.fil | Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina | |
dc.subtype | cientifico |
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