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A successful vitrification technique for goat morulae conservation
(Elsevier, 2022-04-01)The aim of this study was to evaluate the effect of different vitrification and warming processes on the in vitro embryo survival of caprine morulae, considering the day of recovery. A total of 136 morulae of Criolla-Neuquina ... -
Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
(Wiley, 2015-11)In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected ... -
Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality
(Elsevier, 2020-09)Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs. We evaluated the extent of cholesterol and desmosterol incorporation ... -
Desmosterol Incorporation Into Ram Sperm Membrane Before Cryopreservation Improves in vitro and in vivo Fertility
(Frontiers Media, 2021-06)Pregnancy rates in ewes are markedly low after cervical insemination with frozen-thawed sperm. Sensitivity of ram sperm to freeze-thawing is related to the lipid composition of the membrane, particularly to its low sterol ... -
Development of an analytical method to determine malondialdehyde as an oxidative marker in cryopreserved bovine semen
(Royal Society of Chemistry, 2015)Frozen sperm is widely used in artificial insemination of cattle as well as other animal species. As a consequence of the freezing and thawing processes of semen, an excess of reactive oxygen species (ROS) are formed. ROS ... -
Efecto del método de colecta de semen y plasma seminal sobre la supervivencia posdescongelación de espermatozoides ovinos
(Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 2012)Los objetivos del presente trabajo fueron determinar las diferencias en los eyaculados obtenidos con electroeyaculador (EE) y vagina artificial (VA) y evaluar el efecto del agregado de plasma seminal (PS) obtenido con EE ... -
Free cholesterol and cholesterol esters in bovine oocytes: Implications in survival and membrane raft organization after cryopreservation
(2017)Part of the damage caused by cryopreservation of mammalian oocytes occurs at the plasma membrane. The addition of cholesterol to cell membranes as a strategy to make it more tolerant to cryopreservation has been little ... -
Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology
(Elsevier, 2016-01)During the last decades fundamental and applied aspects of mammalian ram sperm cryopreservation have been increasingly explored by scientists and biotechnologists. Many works report modifications in the composition of the ... -
Long-term preservation of Lotus tenuis adventitious buds
(Springer, 2018-11-14)Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves ... -
Mejoramiento cualitativo de espermatozoides ovinos criopreservados a partir de la utilización de moléculas diferenciales de plasma seminal obtenidas por dos métodos de colecta seminal: electroeyaculador y vagina artificial
(Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 2018-03)La aplicación de la inseminación artificial (IA) en cualquier especie requiere del mantenimiento del semen en condiciones no fisiológicas. En ovinos, la IA con semen refrigerado se encuentra más difundida que con semen ... -
¿Por qué y para qué producir embriones bovinos in vitro?
(Estación Experimental Agropecuaria Balcarce, INTA, 2022-07)Nuevo récord mundial de embriones bovinos producidos in vitro promueve un giro hacia el uso de la criopreservación asociada al incremento sostenido en la producción de estos embriones. -
PUFA-rich phospholipid classes and subclasses of ram spermatozoa are unevenly affected by cryopreservation with a soybean lecithin-based extender
(Elsevier, 2022-04)Cryopreservation is known to affect spermatozoa structure and function. Ram sperm are among the most highly sensitive mammalian gametes to freezing, due to their lipid composition, which limit their efficiency in artificial ... -
Recent progress in bovine In vitro‐derived embryo cryotolerance: impact of In vitro culture systems, advances in cryopreservation and future considerations
(Wiley, 2020-03-07)Cryopreservation of in vitro‐derived bovine embryos is a crucial step for the widespread reproduction and conservation of valuable high merit animals. Given the current popularity of bovine in vitro embryo production (IVP), ...