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Mostrando ítems 1-5 de 5

    • Assessing Tn5 and sleeping beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes 

      Bevacqua, Romina Jimena; Fernández y Martin, Rafael; Canel, Natalia Gabriela; Gibbons, Alejandro Eduardo; Texeira, D.I.A.; Lange, F.; Vans Landschoot, Geraldina; Savy, Virginia; Briski, Olinda; Hiriart, María Inés; Grueso, Esther; Ivics, Zoltán; Taboga, Oscar Alberto; Kues, Wilfried A.; Ferraris, S.R.; Salamone, Daniel Felipe (2017-03-16)
      Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains ...

    • Baculovirus transduction in mammalian cells is affected by the production of type I and III interferons, which is mediated mainly by the cGAS-STING pathway 

      Amalfi, Sabrina; Molina, Guido Nicolas; Bevacqua, Romina Jimena; Lopez, Maria Gabriela; Taboga, Oscar Alberto; Alfonso, Victoria (American Society for Microbiology, 2020-11)
      The baculovirus Autographa californica multiple nucleopolyhedrovirus is an insect virus with a circular double-stranded DNA genome, which, among other multiple biotechnological applications, is used as an expression vector ...

    • DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality 

      Ynsaurralde Rivolta, Amanda Eugenia; Suvá, Mariana; Luchetti, Carolina Griselda; Bevacqua, Romina Jimena; Munilla, Sebastian; Rodriguez-Alvarez, Lleretny; Velasquez, Alejandra; Briski, Olinda; Lombardo, Daniel; Salamone, Daniel (Elsevier, 2020-05)
      The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) ...

    • Efficient edition of the bovine PRNP prion gene in somatic cells and IVF embryos using the CRISPR/Cas9 system 

      Bevacqua, Romina Jimena; Fernandez-Martín, R.; Savy, Virginia; Canel, Natalia Gabriela; Gismondi, Maria Ines; Kues, Wilfried A.; Carlson, Daniel F.; Fahrenkrug, S.C.; Niemann, H.; Taboga, Oscar Alberto; Ferraris, Sergio; Salamone, Daniel Felipe (2016-11)
      The recently developed engineered nucleases, such as zinc-finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease ...

    • Transgénesis: una moderna biotecnología reproductiva en animales de interés zootécnico 

      Gibbons, Alejandro Eduardo; Bevacqua, Romina Jimena; Fernández y Martin, Rafael; Pereyra Bonnet, Federico Alberto; Bruno Galarraga, María Macarena; Salamone, Daniel Felipe; Cueto, Marcela Isabel (Gerencia de Comunicación e Imagen Institucional, DNA SICC, INTA, 2014-08)
      Los continuos avances en el conocimiento de la biología molecular han permitido un gran progreso de la ciencia, mediante la modificación genética tanto de virus, bacterias como de organismos superiores. Estos procedimientos ...