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A thermostable GH8 endoglucanase of Enterobacter sp. R1 is suitable for β- glucan deconstruction
Ontañon, Ornella Mailen; Ghio, Silvina; Marrero Diaz De Villegas, Rubén; Garrido, Mercedes Maria; Talia, Paola Mónica; Fehér, Csaba; Campos, Eleonora (Elsevier, 2019-11)Glycoside hydrolase family 8 (GH8) includes endoglucanases, lichenases, chitosanases and xylanases, which are essential for polysaccharides breakdown. In this work, we studied a thermally stable GH8 from the cellulose ... -
Heterologous production and characterization of a thermostable GH10 family endo-xylanase from Pycnoporus sanguineus BAFC 2126
Niderhaus, Cecilia; Garrido, Mercedes Maria; Insani, Ester Marina; Campos, Eleonora; Wirth, Sonia Alejandra (Elsevier, 2018-04)Xylanases are key enzymes for agricultural biomass saccharification for the production of cellulosic ethanol. Success in enzymatic lignocellulose bioconversion is restricted by enzyme production costs, activity and stability ... -
Insights into the xylan degradation system of Cellulomonas sp. B6 : biochemical characterization of rCsXyn10A and rCsAbf62A
Garrido, Mercedes Maria; Piccinni, Florencia Elizabeth; Landoni, Malena; Peña, María Jesús; Topalian, Juliana; Couto, Alicia; Wirth, Sonia Alejandra; Urbanowicz, Breeanna Rae; Campos, Eleonora (Springer, 2022)Valorization of the hemicellulose fraction of plant biomass is crucial for the sustainability of lignocellulosic biorefineries. The Cellulomonas genus comprises Gram-positive Actinobacteria that degrade cellulose and other ... -
Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
Ontañon, Ornella Mailen; Bedő, Soma; Ghio, Silvina; Garrido, Mercedes Maria; Topalian, Juliana; Jahola, Dóra; Fehér, Anikó; Valacco, Maria Pia; Campos, Eleonora; Fehér, Csaba (Springer, 2021-05)One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still ... -
PsAA9A, a C1-specific AA9 lytic polysaccharide monooxygenase from the white-rot basidiomycete Pycnoporus sanguineus
Garrido, Mercedes Maria; Landoni, Malena; Sabbadin, Federico; Valacco, María Pía; Couto, Alicia; Bruce, Neil Charles; Wirth, Sonia Alejandra; Campos, Eleonora (Springer, 2020-09)Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a ... -
Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversio
Ghio, Silvina; Bradanini, María B.; Garrido, Mercedes Maria; Ontañon, Ornella Mailen; Piccinni, Florencia Elizabeth; Marrero Diaz De Vill, Rubén; Talia, Paola Mónica; Campos, Eleonora (Elsevier, 2020-12)In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase ...