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Evaluation of PMA‐qPCR methodology to detect and quantify viable Shiga toxin‐producing Escherichia coli in beef burgers
Rey, Maria De Los Angeles; Cap, Mariana; Favre, Leonardo Cristian; Rodriguez, Anabel; Dus Santos, Maria Jose; Vaudagna, Sergio Ramon; Mozgovoj, Marina Valeria (2021-02-06)Propidium monoazide (PMA) is a selective nucleic acid intercalating dye that can be combined with real‐time PCR (qPCR) in order to evaluate cell viability in food samples. The aim of the present work was to evaluate PMA‐qPCR ... -
High-pressure processing treatment of beef burgers: Effect on Escherichia coli O157 inactivation evaluated by plate count and PMA-qPCR
Rey, Maria De Los Angeles; Rodriguez, Anabel; Rossi Ribeiro, Luma; Dos Santos Cruz, Fabiano; Cap, Mariana; Mozgovoj, Marina Valeria; Cristianini, Marcelo; Vaudagna, Sergio Ramon (Wiley, 2022-04-20)Propidium monoazide coupled to real time PCR (PMA-qPCR) is a novel methodology proposed for the quantification of viable bacteria in food after microbial inactivation treatments. The aim of this work was to assess the ... -
Use of propidium monoazide coupled with real-time PCR methodology to evaluate Escherichia coli O157 lethality in beef burgers after organic acid addition
Rey, María De Los Ángeles; Cap, Mariana; Favre, Leonardo Cristian; Vaudagna, Sergio Ramon; Mozgovoj, Marina Valeria (Institute of Food Technologists (IFT), 2025-03)In order to achieve bacterial inactivation in beef burgers, one of the alternatives proposed is to add organic acids as part of their formulation. The aim of this work was to evaluate the effectiveness of propidium monoazide ...